克氏原螯虾弗氏柠檬酸杆菌的分离鉴定与药敏试验  

Isolation,identification and antibiotics susceptibility test of Citrobacter freundii from Procambarus clarkii

作　　者：陈红莲[1] 宋光同[1] 何吉祥[1] 侯冠军[1] 王永杰[1] 

机构地区：[1]安徽省农业科学院水产研究所,合肥230031

出　　处：《淡水渔业》2014年第1期73-77,共5页Freshwater Fisheries

基　　金：安徽省水产(虾蟹类)产业技术体系;安徽省农业科学院院长青年创新基金项目(12B0515);安徽省农业科学院引进人才科研启动资金

摘　　要：为确定引起养殖克氏原螯虾(Procambarus clarkii)患病的病原种类及耐药情况,从患病克氏原螯虾肝胰腺取样进行常规细菌学检查,对分离株进行回归感染试验、形态观察、生理生化特性检测、16S rRNA和gyrB基因分析及药敏试验。结果显示:从患病克氏原螯虾肝胰腺分离到大量菌落形态和色泽一致的细菌,代表菌株X120523为弗氏柠檬酸杆菌(Citrobacter freundii),具有较强致病性,对20种抗生素中的喹诺酮类和氨基糖苷类抗生素敏感。This experiment was conducted to clarify species and drug resistance of pathogen from the diseased Procambarus clarkia.Pathogenic bacteria from hepatopancreas of the diseased Procambarus clarkia were examined using conventional methods and isolated.The further tests and analysis of the isolated strain were developed , including the regression experi-ment to Procambarus clarkii, the morphology, physiological and biochemical characteristics , sequence analysis of their 16S rRNA and gyrB genes , and the susceptibility test to antibiotics.Large colonies with similar morphology and color were obtained.Strain X120523 was identified as Citrobacter freundii, proved to have strong pathogenicity , and susceptible to quinolones and aminoglycosides.

关 键 词：克氏原螯虾(Procambarus clarkii) 弗氏柠檬酸杆菌(Citrobacter freundii) 分离鉴定 16S RRNA基因 gyrB基因 药敏试验 

分 类 号：S945.492[农业科学—水产养殖]