一株大鳍鳠肠道益生芽孢杆菌的筛选与鉴定  

Screening and identification of a Bacillus for probiotics from Mystus macropterus intestine

作　　者：但言 马跃岗 朱杰 陈元坤 曾东[2] 谭占坤[3] 

机构地区：[1]重庆市水产科学研究所,重庆401121 [2]四川农业大学动物医学院,四川雅安625014 [3]西藏大学农牧学院,西藏林芝860000

出　　处：《淡水渔业》2014年第1期90-93,共4页Freshwater Fisheries

基　　金：重庆市基本科研业务费计划项目(2012-csfc-jbky-00405)

摘　　要：从健康大鳍鳠(Mystus macropterus)肠道菌群分离得到25株细菌,通过对菌株胞外酶活力及对鱼类常见病原菌抑制作用进行筛选,从中筛选出一株益生菌DQHY-7菌株。对DQHY-7菌株安全性进行检测,并结合菌株形态观察、生理生化试验,对DQHY-7菌株进行了初步鉴定。结果显示:DQHY-7菌株具有较强的分泌纤维素酶、淀粉酶和蛋白酶能力,能够抑制大肠杆菌(Escherichia coli)、嗜水气单胞菌(Aeromonas hydrophila)和荧光假单胞菌(Pseudomonas fluorescens)的生长。急性毒性试验表明,浓度为1.0×108CFU/mL的DQHY-7菌悬液对大鳍鳠没有致病性。结合DQHY-7菌株菌落生长形态和生理生化特性,初步鉴定为枯草芽孢杆菌(Bacillus subtilis)。研究结果表明DQHY-7菌株可以作为一个新的饲用微生态制剂候选菌种。25 bacterial strains isolated from intestinal tract of Mystus macropterus were used for testing the ectoenzyme -producing ability and the antagonistic effects against three common pathogenic bacteria .The toxicity of strain DQHY -7 screened as potential probiotic for M.macropterus was evaluated.DQHY-7 was identified according to its morphological , physiological and biochemical characteristics.The results indicated that DQHY -7 had high ectoenzyme -producing ability including amylase , cellulose and protease , and DQHY -7 could produce chemical inhibitor to Escherichia Coil, Aero-monas hydrophila and Pseudomonas fluorescens.It was non-pathogenic for M.macropterus when the concentration of DQHY-7 was at 1.0 ＆#215;10 8 CFU/mL.Combined with the physiological and biochemical characteristics , DQHY-7 could be de-terminedas Bacillus subtilis preliminarily.This study had revealed that DQHY -7 would be wsed as a candidate strain of mi-croecological in aquaculture.

关 键 词：大鳍鳠( Mystus macropterus) 芽孢杆菌( BACILLUS spp) 益生菌 筛选 抑菌 

分 类 号：S917.1[农业科学—水产科学]