高效液相色谱-质谱法对癍痧凉茶的化学成分鉴定及指纹图谱研究  

Chemical Components Identification and Fingerprint Analysis of Bansha Herbal Tea Using High Performance Liquid Chromatography Coupled with Mass Spectrometry

作　　者：梁慧[1] 邓洁薇[1] 杨运云[1] 

机构地区：[1]中国广州分析测试中心广东省分析测试技术公共实验室,广东广州510070

出　　处：《分析测试学报》2014年第2期144-149,共6页Journal of Instrumental Analysis

基　　金：广东省科技计划重点项目(2010A030100013)

摘　　要：采用高效液相色谱-飞行时间质谱(HPLC-TOF-MS)和高效液相色谱-离子阱质谱(HPLC-ITMS)对癍痧凉茶进行化学成分分析。研究结果表明,"黄振龙"和"平安堂"两种癍痧凉茶中共含有40种主要化学成分,其中17种通过与文献对照得到鉴定,13种通过与对照品比较保留时间和质谱数据得到确证。同时采用HPLC-IT-MS建立了"黄振龙"和"平安堂"癍痧凉茶的液相色谱-质谱联用指纹图谱,并采用主成分分析对41批次的癍痧凉茶的质量稳定性和一致性进行了评价。主成分分析结果表明,"黄振龙"和"平安堂"癍痧凉茶之间的化学成分和内在质量具有显著性差异,提示配方组成及生产工艺不同,应予以区分。该分析方法快速、高效、可靠,是癍痧凉茶质量控制的有效手段。A high performance liquid chromatography time -of- flight mass spectrometry （HPLC - TOF- MS） and high performance liquid chromatography ion trap mass spectrometric （HPLC -IT- MS） method were used for the identification of constituents of ＂Huangzhenlong（HZL） ＂ and ＂Pingan- tang（PAT） ＂ Bansha herbal tea（BHT）. The results showed that HZL and PAT BHT contained 40 major chemical constituents. By comparing the mass spectrometric data of each constituent with those of literature, 17 of the 40 constituents were identified. Among them, 13 were confirmed by the com- parison of their retention time and MS data with those of reference substances. In addition, chemical fingerprints of HZL and PAT BHT were established by HPLC - IT - MS, and principal component a- nalysis（PCA） was performed to evaluate the quality consistency and stability of 41 batches of BHT samples. PCA results showed that HZL and PAT BHT samples were significantly different, indicating their formulas and the production processes were different. The developed fingerprinting method was fast, efficient and robust for the quality control of BHT products.

关 键 词：癍痧凉茶 化学成分 指纹图谱 质量控制 高效液相色谱一质谱法 

分 类 号：O657.63[理学—分析化学] TQ460.72[理学—化学]