昆山接骨片对体外培养大鼠成骨细胞骨的影响  

STUDY ON THE EFFECT OF “KUNSHAN JIEGU”TABLET ON THE BONE FORMATION OF RAT OSTEOBLASTS

作　　者：陈奕[1] 赵咏芳[1] 张月林 詹红生[2,1] 石庭芬[2,1] 徐宇 

机构地区：[1]江苏省昆山市中医医院,215300 [2]上海中医药研究院骨伤科研究所

出　　处：《中医正骨》2001年第1期5-6,共2页The Journal of Traditional Chinese Orthopedics and Traumatology

摘　　要：为研究昆山接骨片对体外成骨细胞骨形成功能的影响 ,探讨昆山接骨片促进骨折愈合的机理 ,分别用 MTT法观察了含昆山接骨片 号和昆山接骨片 号的药物血清对成骨细胞增殖功能的影响 ,用 PNPP法观察昆山接骨片 号对成骨细胞碱性磷酸酶比活性的影响 ,以及用矿化结节计数法观察了昆山接骨片 号对成骨细胞矿化功能的影响。成骨细胞增殖率测定结果显示 ,昆山接骨片 号组为 0 .0 92± 0 .0 16 ,与对照组比较有显著性差异 (P<0 .0 5 ) ;而 号组为 0 .2 30± 0 .0 2 6 ,与对照组比较无差异。AL P比活性测定结果显示 ,昆山接骨片 号组为 0 .0 34± 0 .0 2 1,对照组为 0 .0 2 6± 0 .0 0 8,两组比较有显著性差异 (P<0 .0 5 ) )。矿化结节计数结果提示 ,昆山接骨片 号组为 2 5 .833± 7.782 ,对照组为 13.333± 6 .15 3,两组比较有显著性差异 (P<0 .0 5 )。综合研究结果表明 ,以活血化瘀药物为主制成的昆山接骨片 号具有促进成骨细胞的增殖、分化与矿化、成骨细胞的骨形成功能 ,从而发挥其促进骨折愈合的作用 ,单纯用螃蟹粉制成的昆山接骨片Objective:To study the effect of “Kunshan Jiegu” Tablet(KJT) on the bone formation of osteoblasts.Methods:MTT method was used to observe the effects of blood serums of KJT I and KJT II on the proliferation of osteoblasts;PNPP method was used to evaluate the effect of KJT I on the specific activity of alkaline phosphatase of osteoblasts;The counting method of mineralization nodules was used to observe the effect of KJT I on osteoblasts' mineralization.Results:The effect of KJT I group on the proliferation of osteoblasts is significantly different from that of the control group,while the result of KJT II is on the contrary.The effect of KJT I group on the activities of osteoblasts and mineralization differs greatly from that of the control group.Conclusion:KJT I could increase proliferation,differentiation and minralization of osteoblasts,thus could inprove the fracture healing.

关 键 词：昆山接骨片 成骨细胞 生理学 药效学 中药 

分 类 号：R285.5[医药卫生—中药学]