不同地区蜂胶原料药的质量评价  

Quality Evaluation of Propolis Contents in Different Regions

作　　者：贾艾玲[1] 郑爱竹 王睿[1] 邱智东[1] 

机构地区：[1]长春中医药大学,吉林长春130117

出　　处：《辽宁中医杂志》2014年第2期316-318,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81274092)

摘　　要：目的:建立不同产地蜂胶的质量评价方法。方法:采用薄层色谱法,以白杨素和高良姜素为对照品,甲苯-乙酸乙酯-甲酸(10∶4∶3)为展开剂,进行定性鉴别研究;采用高效液相色谱法,色谱柱Dikma Technologies(250 mm×4.6mm,5μm);流动相甲醇-0.15%磷酸水溶液(62∶38);流速1.0 mL·min-1;柱温25℃;检测波长268 nm。结果:28个产地蜂胶原料药中白杨素和高良姜素的含量均符合《中国药典》(2010年版)的规定的仅有6个地区,大部分地区蜂胶原料药中高良姜素含量符合标准,而白杨素含量不达标。结论:不同产地蜂胶原料药中白杨素和高良姜素的含量不同,建立的定性、定量方法准确可靠,专属性强,适合不同产地蜂胶原料药的质量控制。Objective：To establish the methods of Propolis content determination from different regions. Methods：Thin layer chromatography （TLC）was adopted, using chrysin and galangin as reference substance and toluene, ethyl acetate -formic acid（ 10 ：4：3 ）as developing agent, to study propolis of different regions. High performance liquid chromatography （HPLC）method was a- dopted,using octadeeyl silane bonded silica as stationary phase. Methanol 0.15% phosphoric acid aqueous solution（ 62：38 ）was as mobile phase ; setting flow rate was 1.0 ml/min, detection wavelength was 268 nm, to determine the contents of chrysin and ga- langin in propolis from different regions. Results ： There were only six areas where the galangin and chrysin met the standards given by Pharmacopoeia of China（2010 version）from 28 samples of different regions. Conclusion：Contents of ehrysin and galangin from propolis of different origins vary. The established quantitative and qualitative methods are accurate, reliable and exclusive, provi- ding a basis for the quality control of propolis and its pharmaceutical preparation.

关 键 词：蜂胶 白杨素 高良姜素 薄层色谱法 高效液相色谱法 

分 类 号：R285[医药卫生—中药学]