耐辐射球菌对Cu^(2+)的去除效率  

Removal efficiency of Cu^(2+) by Deinococcus radiodurans

作　　者：韩万春[1] 李铭锋[2,3] 田兵[2] 华跃进[2] 卢振兰[1] 

机构地区：[1]吉林农业大学资源与环境学院,吉林长春130118 [2]浙江大学原子核农业科学研究所,浙江杭州310029 [3]山东省东营市环境保护局,山东东营257000

出　　处：《水资源保护》2014年第1期64-67,共4页Water Resources Protection

基　　金：国家自然科学基金(30830006;31170079)

摘　　要：耐辐射球菌是一种非致病菌,拥有极强的抗辐射、抗氧化特性,具有成为工程菌处理Cu2+的潜力。采用酶标仪测定Cu2+对耐辐射球菌的生长速度的影响,用电感耦合等离子光谱法测定菌液离心后得到的上清液Cu2+浓度。结果表明,当Cu2+初始质量浓度为1.36 mg/L时,耐辐射球菌对其清除效率为57.3%;当Cu2+初始质量浓度为6.28mg/L时,对其清除效率为35.4%。此外,用激光共聚焦显微镜观察受到Cu2+胁迫的耐辐射球菌,发现球菌具有明显的聚集效应。As a non-pathogenic bacteria, Deinococcus radiodurans is extremely resistant to ionizing radiation and oxidative stress and shows great potential in remediation of Cu2＋ pollution. In this study, we used the ELIASA plate reader and the ICP-OES, respectively, to monitor the growth rate of Deinococcus radiodurans and the Cu2＋ concentration in the supernatant after the bacterial solution was centrifuged. The results show that the removal efficiency of Cu2＋ by Deinococcus radiodurans reached 57.3% when the initial concentration of Cu2＋ was 1.36mg/L, and the removal efficiency dropped to 35.4% when the initial concentration of Cu2＋ increased to 6.28 mg/L. Meanwhile, using confocal laser scanning microscopy （CLSM）, we observed that Deinococcus radiodurans assembled at a high concentration of Cu2＋.

关 键 词：耐辐射球菌Cu2+ 电感耦合等离子光谱法 去除效率 

分 类 号：X703.1[环境科学与工程—环境工程]