睡眠剥夺对冠状动脉粥样硬化大鼠血清白介素-1β水平的影响(英文)  

Influence of sleep deprivation on serum interleukin-1β level in rats with coronary atherosclerosis

作　　者：谭利[1] 黄琪[2] 李德梅[3] 陈玲玲[1] 张雨馨[1] 王治校[1] 

机构地区：[1]湖北医药学院附属太和医院心内科 [2]湖北医药学院基础医学院 [3]湖北医药学院附属人民医院老年病科,湖北十堰442000

出　　处：《心血管康复医学杂志》2014年第1期1-5,共5页Chinese Journal of Cardiovascular Rehabilitation Medicine

基　　金：2010 Scientific research project of Hubei provincial department of education(B20102104)~~

摘　　要：目的：探讨睡眠剥夺（SDP）对冠状动脉粥样硬化（CAS）大鼠血清白介素-1β （IL-1β）水平的影响.方法：选取健康SD大鼠96只,按数字表法随机分为4组：正常对照组、CAS组、SDP组以及SDP合并CAS组,每组各24只；CAS组和SDP合并CAS组喂高胆固醇饲料,建立CAS动物模型；通过小平台水环境法,对SDP组和SDP合并CAS组大鼠进行睡眠剥夺.采用放射免疫方法检测血清IL-1β浓度；并分别测定血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）及低密度脂蛋白胆固醇（LDL-C）含量；于4组间对上述指标进行比较.结果：与正常对照组血清IL-1β浓度比较,SDP组、CAS组和SDP合并CAS组IL-1β水平均显著升高[（13.33±3.20） ng/L vs.（14.40±4.41） ng/L vs.（22.21±5.94） ng/L vs.（30.17±7.45） ng/L] （P＜0.05～＜0.01）;与正常对照组和SDP组比较,CAS组及SDP合并CAS组的TC、TG、LDL-C水平均显著升高,HDL水平均显著降低（P＜0.05～＜0.01）；SDP合并CAS组的上述血脂指标均显著高于或低于CAS组（P均＜0.05）.结论：睡眠剥夺可以显著升高冠状动脉粥样硬化和睡眠剥夺大鼠的血清白介素-1β,TC、TG、LDL-C水平,和降低其HDL水平.Objective：To explore influence of sleep deprivation （SDP） on serum interleukin-1β （IL-1β） level in rats with coronary atherosclerosis （CAS）.Methods：A total of 96 healthy SD rats were randomly and equally divided into normal control group,CAS group,SDP group and SDP ＋ CAS group according to number table; rats in CAS group and SDP ＋ CAS group were fed with high cholesterol diet to establish CAS animal model; SDP group and SDP ＋ CAS group received SDP through small platform with water environment.Radioimmunoassay was used to measure serum concentration of IL-1β; and serum concentrations of total cholesterol （TC）,triglyceride （TG）,high density lipoprotein-cholesterol （HDL-C） and low density lipoprotein-cholesterol （LDL-C） were measured respectively; above indexes were compared among four groups.Results：（1） Compared with serum IL-1β level of normal control group,there were significant increase in SDP group,CAS group and SDP ＋ CAS group [（13.33±3.20） ng/Lvs.（14.40±4.41） ng/L vs.（22.21±5.94） ng/L vs.（30.17±7.45） ng/L] （P〈0.05～〈0.01） ; （2） Levels of TC,TG and LDL-C in CAS group and SDP ＋ CAS group were significantly higher,level of HDL were significantly less than those of normal control group and SDP group （P〈0.05～〈0.01）,levels of above indexes in SDP ＋ CAS group were significantly higher or less than those of CAS group （P〈0.05 all）.Conclusion：Sleep deprivation can significantly heighten serum interleukin-1β level and total cholesterol,triglyceride,low density lipoprotein-cholesterol levels,decrease HDL level in rats with coronary atherosclerosis or sleep deprivation.

关 键 词：冠状动脉硬化 白细胞介素1 睡眠剥夺 大鼠 

分 类 号：R541.409[医药卫生—心血管疾病]