维生素E对高脂血症小鼠Th1和Th2细胞因子表达的影响  

Effect of Vitamin E on Expressions of Th1 and Th2 Cytokines in Mice with Hyperlipidemia

作　　者：霍阳[1] 初巍巍[1] 贾大林[2] 宋涛[1] 陈悦[1] 赵跃萍[1] 

机构地区：[1]武警辽宁省总队医院内一科,沈阳110034 [2]中国医科大学附属第一医院心内科,沈阳110031

出　　处：《临床误诊误治》2014年第2期103-106,共4页Clinical Misdiagnosis & Mistherapy

基　　金：辽宁省科学计划发展项目(20092250511)

摘　　要：目的观察维生素E对高脂血症小鼠辅助性T淋巴细胞1(Th1)和辅助性T淋巴细胞2(Th2)细胞因子表达的影响。方法将50只ICR雄性性成熟小鼠随机分为A、B、C、D、E 5组,每组10只,分别给予高脂饲料、不同浓度维生素E(每日10 mg/kg、20 mg/kg、40 mg/kg)加高脂饲料及普通饲料连续喂养4周,后处死小鼠抽取静脉血观察比较血清Th1和Th2细胞因子表达情况[肿瘤坏死因子-α(TNF-α)、白细胞介素-12(IL-12)及白细胞介素-4(IL-4)、白细胞介素-5(IL-5)]及甘油三酯(TG)、胆固醇(TC)浓度。结果 A组血清TNF-α、IL-12及TG、TC浓度均高于B、C、D、E组,血清IL-4及IL-5浓度均低于B、C、D、E组,差异均有统计学意义(P<0.05)。B、C、D 3组血清TNF-α、IL-12及TG、TC浓度均随维生素E剂量增加逐渐降低,血清IL-4及IL-5浓度随维生素E剂量增加逐渐升高,且3组间比较差异均有统计学意义(P<0.05)。E组血清TNF-α、IL-12及TG浓度均低于B、C、D组,血清TC浓度低于B、C组,血清IL-4及IL-5浓度均高于B、C、D组,除D、E 2组血清IL-4比较差异无统计学意义外(P>0.05),余差异均有统计学意义(P<0.05)。血清IL-5浓度与IL-12浓度呈负相关关系。结论维生素E能抑制高脂血症所导致的免疫损伤与炎性反应,并可降低血脂。Objective To explore the effect of Vitamin E on expressions of helper T cells （Thl and Th2） in mice with hyperlipidemia. Methods A total of 50 viripotent male ICR mice were randomly divided into group A, B, C, D and E （n = 10）. Mice fed on high-fat food in group A, B, C and D, and group B, C and D were lavaged with Vitamin E in different concentrations （10 mg/kg, 20 mg/kg and 40 mg/kg at each day） respectively for 4 weeks, while control group was fed on normal diet for 4 weeks, and then all the mice were sacrificed. The expressions of Thl and Th2 cytokines [ tumor necrosis fac- tor a （TNF-α） , interleukin 12 （IL-12） , interleukin 4 （IL-4） and interleukin 5 （IL-5）] and the concentrations of triglyeer- ide （TG） and cholesterol （TC） in serums of venous blood in the five groups were detected and compared. ReSults The con- centrations of TNF-α, IL-12, TG and TC in group A were significantly higher than those of group B, C, D and E （P 〈 0.05 ） , while the eoncentrations of IL-4 and IL-5 in group A were significantly lower than those of group B, C, D and E （ P 〈 0.05 ）. In group B, C and D, the concentrations of TNF-α, IL-12, TG and TC were decreased gradually with increasing con- centration of Vitamin E, while the concentrations of IL-4 and IL-5 were increased gradually with increasing concentration of Vitamin E, and the differenees among the three groups were statistically significant （P 〈 0.05 ）. In group E, concentrations of TNF-et, IL-12, and TG were lower than those of group B, C and D, TC concentration was lower than those of group B and C, concentrations of IL-4 and IL-5 were higher than those in group B, C and D, and the differences were statistically significant （ P 〈 0.05 ） except for the difference of serum IL--4 concentration between group D and E （ P 〉 0.05 ）. IL-5 concentration was negatively related with the IL-12 concentration. Conclusion Vitamin E can inhibit the immune injury and inflammatory reac- tion induced by hyperlipemia and br

关 键 词：维生素E 高脂血症 Thl细胞因子 TH2细胞因子 

分 类 号：R589.2[医药卫生—内分泌]