Dot-ELISA和RT-PCR检测南方水稻黑条矮缩病毒灵敏度的比较  

Comparison of Sensitivity of Dot-ELISA and RT-PCR in Detecting Southern Rice Black Streaked Dwarf Virus

作　　者：刘珊珊[1] 周倩[1,2] 高必达[1,2] 李有志[1,2] 张政斌[3] 郭海明[3] 

机构地区：[1]湖南农业大学植物保护学院,湖南长沙410128 [2]植物病虫害生物学与防控湖南省重点实验室,湖南长沙410128 [3]湖南省植保植检站,湖南长沙410005

出　　处：《湖南农业科学》2014年第1期52-54,57,共4页Hunan Agricultural Sciences

基　　金：公益性行业(农业)科技专项(201303021);公益性行业(农业)科技专项(201003031);湖南省科技厅项目(2010NK3021)

摘　　要：南方水稻黑条矮缩病毒(Southern rice black-streaked dwarf virus,SRBSDV)是近年来在我国南方稻区和越南危害严重的一种新病毒,主要由白背飞虱传播,造成水稻大面积减产。检测田间白背飞虱、杂草和水稻植株的带毒率是南方水稻黑条矮缩病预测预报的基础。为寻找一种适合大量样本的检测方法,以感染南方水稻黑条矮缩病毒的水稻植株和带毒的白背飞虱为材料,比较了斑点免疫测定法(Dot-ELISA)和反转录-聚合酶链式反应(RT-PCR)两种检测方法的灵敏度。试验结果表明:RT-PCR技术的检测灵敏度比Dot-ELISA技术检测灵敏度高。Southern rice black streaked dwarf virus （SRBSDV）, a new virus, had caused a serious rice dwarf disease in southern China and Vietnam in recent years, which mainly spread by the white backed planthopper （WBPH）, leading to serious reduction of rice production. Detecting the infected rate of WBPH, weeds and rice plants in field is the foundation of forecasting the SRBSDV. To find a detecting method which is suitable for a large number of samples, the SRBSDV infected rice plants and WBPH were taken as the materials to compare the sensitivity of Dot immuno-assay （Dot-ELISA） and reverse transcription - polymerase chain reaction （RT-PCR）. The results showed that the sensitivity of RT-PCR was higher than the sensitivity of Dot-ELISA.

关 键 词：南方水稻黑条矮缩病毒 反转录-聚合酶链式反应 斑点免疫测定 检测灵敏度 

分 类 号：S432.4[农业科学—植物病理学]