瓯江彩鲤早期发育过程中磷酸酶活性及间甲酚对其活性的影响  

Phosphatase Activity and Effects of m-Cresol on Phosphatase Activity during Early Development of Common Carp Cyprinus carpio var.color

作　　者：朱俊华[1] 姚俊杰[1] 冯亚楠[1] 蒋左玉 熊铧龙[1] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025

出　　处：《水产科学》2014年第2期92-96,共5页Fisheries Science

基　　金：国家自然科学基金资助项目(31160527);贵州大学研究生创新基金资助项目(研农2013018)

摘　　要：为探究环境雌激素对鱼类早期发育的毒性作用,采用生化和静态急性毒性试验方法,研究了瓯江彩鲤早期发育过程中碱性磷酸酶和酸性磷酸酶活性的变化及间甲酚对瓯江彩鲤早期发育中磷酸酶活性的影响。试验结果表明,瓯江彩鲤受精卵中磷酸酶活性均低于成熟卵,卵裂期酶活性最低;在水温(20±2)℃条件下,经7、13、19mg/L的间甲酚暴露,各质量浓度组磷酸酶活性表现为良好的剂量—效应和发育时期—效应关系。总体来说,瓯江彩鲤从原肠胚期开始,磷酸酶活性随着发育时间的延长和暴露质量浓度的增加而升高。Activities of acid phosphatase （ACP） and alkaline phosphatase （ALP） were determmect ano the effect of m-cresol on the activities of ACP and ALP was studied during early development of common carp Cyprinus carpio var. color by biochemical and static toxicological methods to evaluate the toxic effect of environmental estrogens on early development of fish. The results showed that the activities of phosphatase were lower in the fertilized eggs than those in the mature eggs, with the minimal value in cleavage stage of the fertilized eggs exposed to the m-cresol at concentration of 7 rag/L, 13 mg/L and 19 mg/L at water temperature of （20 ±2）℃. There was a close dose-effect and development stage-effect relationship for the activities of ALP and ACP in each concentration group. In general, the activities of ALP and ACP were found to be increased with the development and the increase in m-cresol concentration during different early development stages of the common carp.

关 键 词：间甲酚 瓯江彩鲤 早期发育 酸性磷酸酶 碱性磷酸酶 

分 类 号：S965.199[农业科学—水产养殖]