黄翅大白蚁后肠降解滤纸微生物群落的分析  

Analysis of cellulose-degrading microbiome of Macrotermes barneyi hindgut

作　　者：员超[1] 张宁[1] 倪金凤[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100

出　　处：《生物加工过程》2014年第1期13-17,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家重点基础研究发展计划(973计划)(2011CB707402);国家自然科学基金(31272370)

摘　　要：黄翅大白蚁(Macrotermes barneyi)具有高效降解木质纤维素的能力,其后肠内存在着丰富的共生微生物。采用活性电泳和变形梯度凝胶电泳的方法对黄翅大白蚁后肠降解滤纸微生物群落进行分析。活性电泳实验证实了此微生物群落纤维素酶的存在(内切葡聚糖酶、β-葡萄糖苷酶和木聚糖酶),变形梯度凝胶电泳实验鉴定出微生物组的群落结构,即7种细菌和3种真菌。本研究初步阐明了黄翅大白蚁后肠内与滤纸降解相关的微生物种类,为进一步了解黄翅大白蚁纤维素的降解机制以及生物质资源的高效利用提供了理论基础。Macrotermes barneyi hindgut harbored large quantity of microbes.The native-page method and the denatured gradient gel electrophoresis were used to analyze the filter paper-degrading microbiome of M.barneyi hindgut.EG, BG and xyl were verified by the native-page method.Microbial community structure was identified by the denatured gradient gel electrophoresis.The microbial species,including 7 bacterial and 3 fungi,associated with filter paper degradation in the hindgut of M.barneyi were illustrated.The understanding of cellulose degradation mechanisms was facilitated and some theoretical basis for efficient biomass transformation was provided.

关 键 词：黄翅大白蚁 微生物群落 木质纤维素 变性梯度凝胶电泳 

分 类 号：Q939[生物学—微生物学]