超声联合微泡促进骨髓间充质干细胞迁移的实验研究  

Effect of ultrasound mediated microbubbles on migration of bone marrow mesenchymal stem cells

作　　者：范国峰[1] 沙杜鹃[1] 徐鹏[1] 张均[1] 童嘉毅[2] 钱健[3] 李鹏[2] 潘涛[1] 

机构地区：[1]南京大学医学院附属鼓楼医院急诊中心,江苏南京210008 [2]东南大学附属中大医院心脏科,江苏南京210009 [3]南京医科大学鼓楼临床医学院,江苏南京210008

出　　处：《东南国防医药》2014年第1期20-22,共3页Military Medical Journal of Southeast China

基　　金：江苏省卫生厅青年科研课题(Q201306);南京大学医学院附属鼓楼医院青年启动基金(QJ2013002);南京市医学科技发展项目基金(YKK12071)

摘　　要：目的探讨体外超声联合微泡对骨髓间充质干细胞迁移的影响,为超声微泡促进干细胞迁移的体内研究提供依据。方法体外培养大鼠骨髓间充质干细胞,分为超声联合微泡组、单纯超声组、单纯微泡组及空白对照组。超声探头频率1 MHz,强度1 W/cm2,进行脉冲超声辐射,采用transwell小室培养法,观察超声联合微泡对骨髓间充质干细胞运动的影响。结果空白对照组、单纯微泡组骨髓间充质干细胞的迁移能力较低,超声联合微泡组与单纯超声组细胞的迁移能力无显著差异(P>0.05),但两者比空白对照组、单纯微泡组迁移能力有显著差异(P<0.01)。结论超声及超声联合微泡作用能明显增强间充质干细胞的迁移能力。Objective To investigate the effects of ultrasound mediated microbubbles on migration of bone marrow mescnchyreal stem cells （BMSCs）. Methods Bone marrow mesenchymal stem cells were cultured in vitro. The subjects in this study were divided into ultrasound mediated microbubbles group, ultrasound radiation group, microbubbles group and control group. Ultrasound （ frequency 1.0 MHz,intensity 1.0 W/cm2） was used. The effect of ultrasound mediated microbubbles on BMSCs migration was observed by applying transWell Chemotaxis. Results There was no obvious difference in control group and microbubbles group （ P 〉 0.05 ）. Both ultrasound mediated microbubbles and ultrasound radiation group are different from control group and microbubbles group （ P 〈 0. 01 ）. Conclusion ultrasound radiation and ultrasound mediated microbubbles promote the migration of BMSCs.

关 键 词：超声微泡 骨髓间充质干细胞细胞 迁移 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]