长时间现地保存解冻牛细管冻精检测研究  

Detection of Thawed Bovine Frozen Sperm From the In-situ and Long-time-conserved Samples

作　　者：卫喜明[1] 许红喜[1] 张洪涛[1] 宋伟红[1] 韩欢胜[1] 韩志强[1] 甘文平[1] 

机构地区：[1]黑龙江农垦科学院畜牧兽医研究所,黑龙江哈尔滨150038

出　　处：《中国牛业科学》2014年第1期18-20,共3页China Cattle Science

基　　金："现代农业(奶牛)产业技术体系建设专项资金资助CARS-37"~~

摘　　要：[目的]本文为了对条件差的养牛场(现地)解冻的细管冻精能长时间保存。[方法]做了2方面的实验研究:实验1:建立对解冻后的精子活力和细菌数2个关键检测指标方法的建立研究;实验2:设计冰水混合物及室温2种保存环境在不同时间对解冻后精子活力和菌落数的影响。[结果]表明:在室温保存2h精子活力差异不显著,2h后差异显著;解冻后精子在冰水混合物里保存4h活力和细菌数差异不显著,4h后细菌数增多,活力下降,差异显著。[结论]牛场要将解冻后精液保存在放到有冰袋的水里效果更好。[Objective]To study preservation time of the thawed frozen sperm from the in-situ samples in poor cattle farm, quality evaluation of the thawed sperm were investigated. [Methods]Two experiments were designed. Experiment I：two key indicator detection methods was established to study sperm motility and bacterial numbers after thawing. Experiment II：the influence of ice and water mixture and room tem- perature at different time by two kinds of storage environment on frozen-thawed sperm motility and colony number was investigated. [Results] The results showed that there was no significance for sperm motility of thawing sperm in a mixture of ice and water within 2 hours, but there was significantly difference after 4 but there was significant difference after 4 hours. Especially, bacteria count increased sperm motility decreased. [Conclusions]Preserving in ice water for bovine frozen semen after 4 hours, but was better.

关 键 词：牛冷冻精液 精子活力 细菌数 品质检测 

分 类 号：S814[农业科学—畜牧学]