高效液相色谱法测定清热凉血丸中黄芩苷的含量  

Determination of Baicalin in Qingreliangxue Pills by HPLC

作　　者：金佩芬[1] 张关顺[1] 崔波[1] 

机构地区：[1]浙江省嘉兴市药品检验所,314001

出　　处：《基层中药杂志》2001年第1期20-21,共2页Primary Journal of Chinese Materia Medica

摘　　要：目的 :建立清热凉血丸中黄芩苷的含量测定项。方法 :超声提取 ,使用 C1 8柱 ,甲醇 -水 -磷酸 (47:5 3:0 .3)为流动相 ,检测波长为 2 76 nm。结果 :此方法线性关系良好 ,平均加样回收率为 98.9%。结论 :本方法分离良好 ,可用于清热凉血丸的质量控制。OBJECTIVE:To establish the method of determination of the content of Baicalin in Qingreliangxue pills.METHODS:The samples are extracted by ultrasonic,then Baicalin in Qingreliangxue pills is determined by HPLC.Using C 18 column,with methanol water phosphosic acid (47:53:0.3)as mobile phase and detection at 276 nm.RESULTS:The linearity of this method is well with the recovery of the added sample is 98.9%.CONCLUSION:This method shows good separating degree and can be used for control quality of Qingceliangxue pills.

关 键 词：高效液相色谱 黄芩苷 清热凉血丸 中药 含量 

分 类 号：R286.1[医药卫生—中药学]