红花性状标记杂交棉新品种鲁05H9 SSR指纹图谱构建及应用  

Construction and application of SSR fingerprints of hybrid cotton variety Lu 05H9 with red-corolla marker trait

作　　者：赵军胜[1,2] 王秀丽[1,2] 高明伟[1,2] 王家宝[1,2] 王永翠[1,2] 陈莹[1,2] 姜辉[1,2] 杨静[1,2] 王留明[1,2] 

机构地区：[1]山东大学农学院,山东济南250100 [2]山东棉花研究中心,山东济南250100

出　　处：《山东大学学报（理学版）》2014年第1期44-49,共6页Journal of Shandong University(Natural Science)

基　　金：国家棉花产业技术体系资助项目(优质育种岗位CARS-18-10);国家高技术研究发展计划资助项目(强优势棉花杂交种的创制与应用2011AA10A102);山东省农业良种工程资助项目(2012LZ05-03)

摘　　要：利用SSR分子标记技术对红花标记杂交棉新品种鲁05H9及其亲本材料进行多态性检测,从多态性高、重复性好的98对SSR核心引物中筛选出12对在双亲间具有多态性的引物、在F1中表现为杂合带的共显性标记。利用该12对引物构建了鲁05H9及其亲本的SSR指纹图谱。利用核心引物组合扩增的方法,可以鉴定鲁05H9的真实性和纯度,对于品种保护、纯度鉴定及杂交种推广具有极其重要的意义。SSR markers were used to analyze the fingerprinting of hybrid cotton variety Lu 05H9 with red-corolla mark- er trait. Lu 05H9 and its parents were amplified with 98 pairs of core primers with high polymorphisms and good repeat- ability. Out of them, 12 paris were detected to be polymorphic between the two parents, which were codominant mark- ers since they amplified the different fragments between the two parents and heterozygosis bands in F1. Fingerprints of Lu05H9 were constructed with the amplification result of the 12 paris primers. It provides a method for seed genuineness and purity detection of hybrids. Due to its fast readability and large storage capacity, DNA fingerprinting of Lu05H9 is in favor of commercial applications.

关 键 词：杂交棉 红花标记性状 SSR 指纹图谱 

分 类 号：S562.035[农业科学—作物学]