子宫平滑肌大电导钙激活钾通道α、β亚基表达与产后出血的关系  

Expression of large conductance Ca^2+activated K^+channel in postpartum hemorrhage

作　　者：颜建英[1] 廖秋萍[1] 刘青闽[1] 徐榕莉[1] 黄晓燕[1] 林顺和[1] 陈文祯[1] 罗美瑜[1] 

机构地区：[1]福建医科大学福建省妇幼保健院教学医院,福建福州350001

出　　处：《中国实用妇科与产科杂志》2014年第2期113-117,共5页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：福建省科技计划重点项目(2009Y0008);福建省医学创新课题(2009-CXB-32)

摘　　要：目的探讨产妇子宫平滑肌大电导钙激活钾通道(BKCa)α、β亚基表达变化在宫缩乏力性产后出血发病中的作用。方法 2009年10月至2011年2月福建省妇幼保健院产科,采用肌条等长张力实验测定30例宫缩乏力性产后出血产妇(病例组)和30例宫缩正常无产后出血产妇(对照组)子宫平滑肌收缩频率、幅度及收缩活动力;应用蛋白印迹法(Western blot)测定两组产妇子宫平滑肌BKCa通道α、β亚基蛋白表达水平;应用实时荧光定量聚合酶链反应(real-time RT-PCR)测定两组产妇子宫平滑肌BKCa通道α、β亚基mRNA表达水平。结果 (1)病例组子宫平滑肌自发宫缩收缩频率、幅度、收缩活动力均低于对照组,分别比较差异均有统计学意义(P<0.05)。(2)病例组子宫平滑肌BKCa通道α、β亚基蛋白表达水平及其mRNA表达水平均高于对照组,分别比较,差异均有统计学意义(P<0.05)。(3)两组产妇子宫平滑肌收缩活动力与BKCa通道α、β亚基蛋白表达水平及BKCa通道α、β亚基mRNA表达水平均呈明显负相关(病例组:r分别为-0.401、-0.727及-0.723、-0.895;对照组:r分别为-0.541、-0.378及-0.703、-0.625;P均<0.05)。结论宫缩乏力性产后出血产妇子宫平滑肌BKCa通道α、β亚基表达水平升高可能是发病的重要因素之一。Objective To detect the expression of large conductance Ca2＋ activated K ＋ channel（BKCa） in myometrium in patients with postpartum hemorrhage（PPH）. Methods The levels of contractile frequency, amplitude and contraction of myometrium were detected by RM6240 biological experimental system ; the BKCa （ α,β subunit） protein expression levels in myometrium were detected by Western blot; the BKCa（ α,β subunit） mRNA expression levels in myometrium were detected by real-time fluorescent quantitative reverse transcription-polymerase chain reaction for women with PPH caused by uterine atony（ study group, n = 30） and normal uterine smooth muscle contraction（ control group, n = 30）. Resuits （ 1 ） In study group, the levels of myometrium contractile frequency, amplitude and contraction were lower than that of the controls（P 〈 O. 05）. （2） In study group, BKCa （α,β subunit） protein and mRNA expression in myometri- um were significantly higher than that of control group（ P 〈 0. 05 ）. （ 3 ） Both groups, the level of myometrium contraction was negatively related to BKCa （α,β subunit） protein, BKCa （ α,β subunit） mRNA level in myometrium. Conclusion Increased BKCa expression in myometrium may be involved in the pathogenesis of PPH caused by uterine atony.

关 键 词：产后出血 宫缩乏力 大电导钙激活钾通道 

分 类 号：R714.461[医药卫生—妇产科学]