Foxp3^+T细胞与人乳头瘤病毒58型基因组整合在宫颈病变发展中的关系  

Expression of Foxp3 and integration of human papillomavirus 58 genome in the progression of cervical lesions

作　　者：朱佳妮[1] 周佳任[1] 张晶[1] 马宁耶[1] 张瑶[1] 常晓晗[1] 丁爱萍[1] 张淑兰[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,辽宁沈阳110004

出　　处：《中国实用妇科与产科杂志》2014年第2期139-143,共5页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：国家自然科学基金(81372776);辽宁省医学高峰建设工程项目(2010696);辽宁省科学技术计划项目(2011225009);盛京自由研究者计划(200806);沈阳市科学技术计划项目(F11-262-9-15)

摘　　要：目的通过检测不同宫颈病变组织中Foxp3+调节性T细胞(regulatory T cell,Treg)的表达及人乳头瘤病毒(human papilloma virus,HPV)基因组整合状态,研究Treg与人乳头瘤病毒整合状态在宫颈病变发病机制中的作用及意义。方法选取2012年11月至2013年7月间,中国医科大学附属盛京医院妇产科住院的119例单一HPV58阳性患者的宫颈脱落细胞标本及相应宫颈组织石蜡标本。包括正常宫颈22例、宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)79例、宫颈癌(cervical cancer,CC)18例。多重PCR技术同时检测HPV58型E2和E6基因,通过E2/E6比值法评估HPV58型基因组在不同宫颈病变中的整合状态。免疫组化方法检测宫颈组织中Foxp3表达情况。结果 HPV58的总整合率为81.51%,HPV58的整合率在正常组中为72.73%,CINⅠ组为76.19%,CINⅡ组为81.82%,CINⅢ组为88.00%,CC组为88.89%。HPV58的整合率随宫颈病变加重呈逐渐升高,差异有统计学意义(χ2=8.399,P=0.004,线性趋势χ2检验)。Foxp3表达强度与宫颈病变严重程度呈正相关,差异有统计学意义(r s=0.538,P=0.000,Spearman等级相关分析)。Foxp3表达强度在HPV不同整合状态下是不同的,差异有统计学意义(χ2=12.058,P=0.002,Kruskal Wallis检验)。结论 HPV58基因组的整合与Foxp3+Treg在宫颈病变发展中密切相关。Objective To study the relationship between integration of HPV genome and Foxp^3＋ Treg in the progression of cervical lesions. Methods One hundred and nineteen HPV58 positive cervical specimens were collected, including 22 normal cervical samples, 79 CIN and 18 cervical cancer. Multiplex Polymerase Chain Reaction （ Multiplex PCR） was applied to detect the physical status of HPV58 genome. The SP immunohistochemical method was used to detect the expressions of Foxp3 in cervical lesions. Results The integration frequency of HPV58 was 81.51%. The integration frequency of HPV58 for normal group, CIN Ⅰ , CIN Ⅱ , CIN Ⅲ, CC group was 72.73% , 76. 19% , 81.82% , 88.00% , 88.89% respectively. The integration frequency of HPV58 showed an increasing trend in different cervical lesions（ X^2 = 8. 399,P = 0.004, chi-squared value for linear trend in proportions）. The expression of Foxp3 had a positive correlation with the degree of cervical lesion（ rs = 0. 538 ,P = 0. 000, Spearmang rank correlation analysis）. The expression of Foxp3 was significantly different among different cervical lesions. The strength of Foxp3 expression enhanced with integration of HPV genome （X^2 = 12. 058,P =0.002,Kruskal Wallis test）. Conclusion There are close relationships of HPV 58 genome integration correlates with the Foxp3 ＋ Treg in the progression of cervical lesion.

关 键 词：宫颈病变 HPV58 病毒整合 调节性T细胞 FOXP3 

分 类 号：R711.74[医药卫生—妇产科学]