血瘀证与血管内皮细胞内游离钙浓度关系的实验研究  

EXPERIMENTAL STUDY ON CONNECTION OF BOLLD STASIS SYNDROME AND [Ca^(2+)]_i IN VASCULAR ENDOTHELIAL CELL

作　　者：陈利国[1] 孙兆贵[2] 王蕾[2] 陈贵海[2] 贾振华[2] 毛淳[2] 李兰[2] 

机构地区：[1]暨南大学,广东广州510632 [2]山东中医药大学,山东济南250014

出　　处：《山东中医药大学学报》2001年第1期55-57,共3页Journal of Shandong University of Traditional Chinese Medicine

基　　金：山东省教育厅资助项目(J97K15)

摘　　要：Obuective:To research the connection of blood stasis syndrome and [Ca\+\{2+\}]\-i in vascular endothelial cell(VEC) and observe the influence of different compound recipes(Huoxue Huayu,Yiqi Huoxue,Liqi Huoxue) on [Ca\+\{2+\}]\-i in VEC.Method:The rat models were established successfully through feeding them high-cholesterol and high-fat foods.All rats were divided into 6 groups.The model groups were treated by different medinines which were nifedipine,the recipe of Huoxue Huayu,the recipe of Yiqi Huoxue and the recipe of Liqi Huoxue.The blood was taken,and sero-[Ca\+\{2+\}],[TC],[TG]were estimated.[Ca\+\{2+\}]\-i was fluoremetried by means of Fura-2/AM.Result:①Sero-[Ca\+\{2+\}] in model groups was higher than that in the control group(P<0 05).②There was significant differece in raising [Ca\+\{2+\}]\-i in serum between the Chinese medicine groups and the nifedipine group (P<0.05).③[Ca\+\{2+\}]\-i in VEC of the model group was higher than that of the control group.The recipe of Yiqi Huoxue could make [Ca\+\{2+\}]\-i even lower than the others treated by the other recipes.There was significant difference comparing to model group(P<0.05).Conclusion:①When the bolld stasis syndrome happened,[Ca\+\{2+\}]\-i in VEC of rats was higher than that of the normal rats.It also showed that the occurrence of blood stasis was probably due to the reduce of endothelial cell’s barrier function.②The recipe of Yiqi Huoxue could reduce [Ca\+\{2+\}]\-i ,so it had the protective effection on the endothelial cell. [Obuective:To research the connection of blood stasis syndrome and [Ca\+\{2+\}]\-i in vascular endothelial cell(VEC) and observe the influence of different compound recipes(Huoxue Huayu,Yiqi Huoxue,Liqi Huoxue) on [Ca\+\{2+\}]\-i in VEC.Method:The rat models were established successfully through feeding them high-cholesterol and high-fat foods.All rats were divided into 6 groups.The model groups were treated by different medinines which were nifedipine,the recipe of Huoxue Huayu,the recipe of Yiqi Huoxue and the recipe of Liqi Huoxue.The blood was taken,and sero-[Ca\+\{2+\}],[TC],[TG]were estimated.[Ca\+\{2+\}]\-i was fluoremetried by means of Fura-2/AM.Result:①Sero-[Ca\+\{2+\}] in model groups was higher than that in the control group(P<0 05).②There was significant differece in raising [Ca\+\{2+\}]\-i in serum between the Chinese medicine groups and the nifedipine group (P<0.05).③[Ca\+\{2+\}]\-i in VEC of the model group was higher than that of the control group.The recipe of Yiqi Huoxue could make [Ca\+\{2+\}]\-i even lower than the others treated by the other recipes.There was significant difference comparing to model group(P<0.05).Conclusion:①When the bolld stasis syndrome happened,[Ca\+\{2+\}]\-i in VEC of rats was higher than that of the normal rats.It also showed that the occurrence of blood stasis was probably due to the reduce of endothelial cell's barrier function.②The recipe of Yiqi Huoxue could reduce [Ca\+\{2+\}]\-i ,so it had the protective effection on the endothelial cell. [

关 键 词：血瘀证 益气活血 血管内皮细胞 游离钙 实验研究 

分 类 号：R255.7[医药卫生—中医内科学]