抗氧化剂保护少弱精子离心过程氧化应激损伤的实验研究  

Antioxidant's protection of OAS from oxidative stress injury in centrifugal process

作　　者：石明华[1] 李慕军[1] 江莉[1] 黄世扬[1] 黄愿静 张悦[1] 以善佳[1] 

机构地区：[1]广西医科大学第一附属医院生殖医学研究中心,南宁530021

出　　处：《中国性科学》2014年第1期9-11,共3页Chinese Journal of Human Sexuality

基　　金：2013年广西研究生教育创新计划资助项目(YCSZ2013036)

摘　　要：目的:探讨抗氧化剂谷胱甘肽、左卡尼汀对少弱精子体外离心过程中抗氧化应激损伤的作用。方法:按WHO标准选取38份少弱精子样本,每份样本各取1350μL,分为对照组、谷胱甘肽组和左卡尼汀组,各450μL。对照组仅加入EBSS平衡液,谷胱甘肽组加入含有一定水平谷胱甘肽的EBSS平衡液,左卡尼汀组加入左卡尼汀的EBSS平衡液,检测三组活性氧(ROS)、丙二醛(MDA)及精子DNA断裂指数(DFI)并进行比较、分析。结果:三组组间比较均有统计学意义(F活性氧=9.45、P=0.000;F丙二醛=15.79,P=0.000;F DFI=13.56,P=0.000,P均<0.05);两两比较示谷胱甘肽组与左卡尼汀组均较对照组间的活性氧、丙二醛及DFI水平明显降低,差异有统计学意义(P均<0.05));而谷胱甘肽组及左卡尼汀组间的比较均无统计学意义(P均>0.05)。结论:在精液离心前添加一定浓度的左卡尼汀或谷胱甘肽可减少离心过程中产生的过量活性氧对精子的氧化应激性损伤,从而提高精子质量。Objectives： To explore the protection role of L - carnitine for oligo - asthenic sperms （OAS） during in - vitro process of centrifugation. Method： According to the WHO standard, 38 OAS samples were selected and 1350 p^L was taken from each sample. The samples were divided into the control group, GSH group and L - carnitine group, with 450 ixL in each group. In the control group, only EBSS balanced solution was added. In the GSH group, EBSS was added with a certain amount of concentration of GSH. In the L - carnitine group, EBSS was added with certain concentration of L - carnitine. After centrifugation at 1 200r/min for 10 minutes, mal on dialdehyde （MDA） ,reactive oxygen species （ROS） and DNA fracture indexes were detected and compared in the three groups. Results： The results in the three groups showed significant statistical differences （ FROS = 9.45, P = 0. 000 ; FMDA = 15.79, P = 0. 000 ; FDFI = 13.56, P = 0. 000 ; P 〈 0. 05）. The ROS, MDA and DFI levels in the GSH group and L -carnitine group were significantly lower than that in the control group （P 〈 0.05 ）. However, the results showed no significant difference between the GSH group and L - carnitine group （P 〉 0. 05 ） . Conclusion： Adding a certain amount of concentration of GSH or L - carnitine before semen centrifugation can reduce the production of the excessive reactive oxygen species , which can result in oxidative stress damage and improve the sperm quality.

关 键 词：左卡尼汀 谷胱甘肽 离心 氧化应激 少弱精子症 

分 类 号：R167[医药卫生—公共卫生与预防医学]