耳缘静脉放血对偏头痛家兔模型PAG区G蛋白的影响  

Effect of Ear-edge Vein Bloodletting on G Protein in PAG of Migraine Rabbit Models

作　　者：杨佃会[1,2] 阚俊祯[3] 马祖彬[1,2] 韩晶[1,2] 

机构地区：[1]山东中医药大学附属医院,山东济南250011 [2]单秋华全国名老中医药专家传承工作室,山东济南250011 [3]山东中医药大学第二附属医院,山东济南250001

出　　处：《山东中医药大学学报》2014年第1期56-57,61,共3页Journal of Shandong University of Traditional Chinese Medicine

基　　金：山东省自然科学基金(编号:ZR2009CM058)

摘　　要：目的:观察耳缘静脉放血对偏头痛家兔模型中脑导水管周围灰质(PAG)G蛋白含量的影响,初步揭示耳缘静脉放血的中枢镇痛调控机制。方法:30只新西兰家兔按随机数字表法分为三组,分别为耳缘静脉放血组、模型组和空白组。采用硝酸甘油复制偏头痛家兔模型,分别在第1天、第4天、第7天造模30 min后耳缘静脉放血。免疫组化法观察家兔模型PAG区G蛋白含量的变化。结果:耳缘静脉放血组家兔模型PAG区G蛋白含量明显降低,与模型组比较有非常显著性差异(P<0.01)。结论:耳缘静脉放血可明显降低偏头痛家兔模型PAG区G蛋白含量,参与镇痛效应。Objective：To observe the effect on G protein in periaqueductal gray（PAG） of the rabbit models of migraine by the therapy of ear-edge vein bloodletting,revealing the central mechanism of analgesic regu-lation. Methods：Total 30 New Zealand rabbits were randomly divided into 3 groups,ear-edge vein bloodlet-ting group,model group and blank group. By subcutaneous injecting nitroglycerin in the rabbits＇ neck,the migraine models were successfully copied. The ear-edge vein blood were letted after 30 minutes of molding, once every 3 days,3 times a course. Then,we observed the expression of G protein in PAG of rabbits by immunohistochemical method. Results ：The expression of G protein in PAG of the ear-edge vein bloodletting group was significantly reduced compared with the model group（P〈0.01 ）. Conclusion：The therapy of ear-edge vein bloodletting has analgesic effect,which can decrease the expression of G protein in PAG of rabbit models of migraine.

关 键 词：耳缘静脉放血 偏头痛 G蛋白 家兔 实验研究 

分 类 号：R245.323[医药卫生—针灸推拿学] R277.772[医药卫生—中医临床基础]