丹参对衰老鼠脑海马神经细胞凋亡作用的研究  

Effect of radix salivae miltiorrhizae on apoptosis of hippocampal cells in aging rats brain

作　　者：朱健[1] 蔡文纬[1] 陈朝婷[1] 成静[1] 张静敏[2] 沈佰华[3] 

机构地区：[1]上海第二医科大学附属第九人民医院老年医学科,上海200011 [2]白求恩医科大学药学院 [3]上海第二医科大学免疫学研究所

出　　处：《中国老年学杂志》2001年第1期46-48,共3页Chinese Journal of Gerontology

基　　金：上海市老年保健科研基金赞助项目!(9641 0 5)

摘　　要：目的　研究丹参对衰老鼠脑海马神经细胞凋亡的作用。方法　用 TUNEL法及流式细胞仪观察衰老鼠的神经细胞凋亡 ,并用免疫组织化学法检测相关基因表达变化。以丹参注射液干预衰老的大鼠模型 ,观察了丹参对衰老鼠神经细胞凋亡的作用。结果　衰老鼠海马细胞有典型的凋亡特征 ,与丹参组比较海马细胞的凋亡率有明显差异 ,衰老鼠 bcl- 2表达下调 ,而 bax的表达量明显增加 ,基因表达变化与流式细胞仪测定的细胞凋亡率相一致。Objective To study the effect of Radix Salivae Miltiorrhizae(SM) on apoptosis of hippocampal cells in senescing rtas brain.Methods To identify the morphological characters and percent of neuronal apoptosis in hippocampus with TUNEL(teminal deoxynuleotidyl transferase mediated nick end labeling) and FACS scan meanwhile determine the expression of apoptosis relative genes by immunohistochemical stainning.After intervening the aging group by SM,the effect of SM on spoptosis of hippocampal cells was observed.Results There was typical apoptotic morphological characters in aging group's neuron cells.The percent of neuronal apoptosis in aging group was much higher than that in SM group.The expression of bcl 2 was decreased and bax was increased in aging group significantly.The percentage of neuronal apoptosis consistented with relative genes expression.Conclusions SM was found to inhibit the apoptosis occurring in aging rats hippocampus obviously.

关 键 词：衰老 丹参 海马神经细胞 细胞凋亡 

分 类 号：R338[医药卫生—人体生理学] R285.5[医药卫生—基础医学]