猪hnRNPK基因启动子的克隆及序列分析  

Cloning and Sequence Analysis of the Promoter Region of Porcine hnRNPK Gene

作　　者：马超锋 梁小娟[2] 王明玉[2] 徐永杰[2] 

机构地区：[1]信阳市动物疫病预防控制中心,河南信阳464000 [2]信阳师范学院生命科学学院,河南信阳464000

出　　处：《信阳师范学院学报（自然科学版）》2014年第1期27-30,共4页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(U1204326);河南省教育厅科学技术研究重点项目(12B230015);信阳师范学院青年骨干教师资助计划项目(2011)

摘　　要：首次克隆了猪hnRNPK基因启动子序列,并进一步对该序列进行了分析.结果显示:该基因启动子约1 kb,与已报道的人的相应序列相似度为78.9%,具有相同的"TCTCGCGAGA"核心启动子序列和转录起始位点.利用在线软件分析发现,猪hnRNPK基因启动子不含TATA盒,而含有CAAT盒的GC富集区,存在两处CpG岛,具有SP1、UCE.2、GCF、EARLY-SEQ1、TTR_inverted_repeat、NGFI-C、EARLY-SEQ1等多种转录因子潜在结合位点,并且具有8种基序结构.The porcine hnRNPK gene promoter were cloned firstly and analysis by the bioinformatics software .The results showed that the length of porcine hnRNPK promoter is about 1 kb, and had 78.9 percent of the similarity com-paring with human hnRNPK promoter , which had the same core promoters ’ sequence of “TCTCGCGAGA” and tran-scriptional start site .The results of bioinformatics analysis showed that no TATA box and two CpG island are found in porcine hnRNPK gene promoter region , and exited many transcription factors-binding sites such as SP1, UCE.2、GCF, EARLY-SEQ1, TTR_inverted_repeat, NGFI-C, and 8 motifs.

关 键 词：猪 启动子 转录因子 

分 类 号：S823.2[农业科学—畜牧学] Q959.842.03[农业科学—畜牧兽医]