补肾健脾化瘀方对去势骨质疏松大鼠血液流变学指标的影响  

Effects of Kidney-tonifying Spleen-strengthening and Removing Blood Stasis Formula on hemorheology in an ovariectomized rat model of osteoporosis

作　　者：魏秋实[1,2] 邓伟民[1] 王海彬[3] 孙伟珊[1] 霍少川[3] 邵玉[1] 谭新[1] 

机构地区：[1]广州军区广州总医院康复科,广州510010 [2]广州军区广州总医院博士后科研工作站,广州510010 [3]广州中医药大学国家重点学科中医骨伤科重点实验室,广州510405

出　　处：《中华中医药杂志》2014年第2期618-620,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.81273778);广东省科技计划省国际合作项目(No.2012B050600026)~~

摘　　要：目的:探讨补肾健脾化瘀方对去势骨质疏松大鼠血液流变学指标的影响。方法:30只7月龄SD雌性大鼠随机分成对照组、模型组、中药低剂量组、中药高剂量组、福美加组。治疗8周后,收集左侧股骨和全血分别行骨矿含量(BMC)、骨密度(BMD)和血液流变学检测。结果:模型组BMC和BMD低于对照组(P<0.05);对照组、中药高剂量组与福美加组BMD高于模型组和中药低剂量组(P<0.05),而对照组、中药高剂量组与福美加组之间无统计学差异。模型组200/s、30/s、3/s、TK、IR、EPT高于对照组(P<0.05,P<0.01),中药高剂量组200/s、30/s、3/s、TK、IR、EPT低于模型组、中药低剂量组和福美加组(P<0.05);TK、IR、EPT与BMD呈负直线关系。结论:去势大鼠存在血流变异常,且部分指标可能与BMD有关,补肾健脾化瘀方可改善血流变异常状态,提高BMC和BMD。Objective： To explore the effects of Kidney-tonifying Spleen-strengthening and Removing Blood Stasis Formula on hemorheology in an ovariectomized rat model of osteoporosis. Methods： 30 female non-pregnant SD rats （7 months） were randomly divided into 5 groups： control group, ovariectomized group, low-dose herb group, high-dose herb group and fumeijia group. After treatment for 8 weeks, the bone mineral density （BMD） and bone mineral content （BMC） were measured by small animals specially dual energy X-ray absorptiometry. And whole blood were detected with the automatical analysis machine of hemorheology. Results： Compared with control group, the BMC and BMD of ovariectomized group were obviously decreased （P〈0.05）. Compared with the ovariectomized group and low-dose herb group, the BMD of high-dose herb group and fumeijia group were obviously increased （P〈0.05）. There was no statistically significant different among control, high-dose herb and fumeijia group. Compared with control group, the level of 200/s, 30/s, 3/s, TK, IR, EPT of ovariectomized group were obviously increased （P〈0.05, P〈0.01）. Compared with other groups, the level of 200/s, 30/s, 3/s, TK, IR, EPT of high-dose herb group were obviously decreased （P〈0.05）. There was a negative linear relationship between BMD and TK, IR, EPT. Conclusion： Hemorheology is abnormal in ovariectomized rat, and some of index is related to BMD. The formula can improve the abnormal status of blood flow, and increase BMD and BMC.

关 键 词：补肾健脾化瘀方 去势大鼠 骨质疏松 血液流变学 

分 类 号：R259[医药卫生—中西医结合]