大鳞副泥鳅粗线期二价染色体分带研究  

Pachytene Chromosome Banding of Paramisgurnus dabryanus

作　　者：常重杰[1] 杜启艳[1] 余其兴[2] 

机构地区：[1]河南师范大学生命科学学院,新乡453002 [2]武汉大学生命科学学院,武汉430072

出　　处：《动物学杂志》2001年第1期23-26,共4页Chinese Journal of Zoology

基　　金：国家自然科学基金! (No.39770 40 7)资助

摘　　要：报道了一种鱼类二价体显带的新方法。采用 pH值在 7 2～ 7 4之间的低渗液对大鳞副泥鳅精巢进行整体长低渗、卡诺固定液处理方法得到了粗线期二价染色体的高分辨G带 ,其带纹特征和数目相对稳定且清晰可辨。与胰酶显带法和EDTA显带法相比具有操作简单、耗时短以及二价体分散相对较好等特点。这一方法在所有显带方法中最为简便。A new method for Pachytene chromosome G banding was reported in this paper.The whole testis was treated with basic hypotonic solution(pH 7.2～7.4) for 4～6 h,then treated with Carnoy's solution for 3～4 times.The cell suspensions were discarded on cold slides.The high resolution G bands of meiotic pachytene chromosome in Paramisgurnus dabryanus was obtained after dyed with Giemsa.The characteristic and numbers of bands were stable and distinct.Compared with trypsin or EDTA treated methods,this protocol is the simplest one with operation simple,less time needed and high rate of success.

关 键 词：大鳞副泥鳅 粗线期染色体 碱性低渗液显带法 分带研究 

分 类 号：Q953[生物学—动物学]