一种动物基因组DNA提取方法的改进  

A New Rapid Method for Extraction of High Quality of Genomic DNA from Animal Tissues

作　　者：汪永庆[1] 王新国[2] 徐来祥 张知彬[1] 

机构地区：[1]中国科学院动物研究所农业害虫害鼠综合治理研究国家重点实验室,北京100080 [2]军事医学科学院生物工程研究所,北京100071 [3]阜师范大学生物学系

出　　处：《动物学杂志》2001年第1期27-29,共3页Chinese Journal of Zoology

基　　金：国家自然科学基金!重点项目 (No .39730 0 90 );中国科学院项目! (KZ95 1 B1 10 6 ;STZ 1 0 5 )资助

摘　　要：介绍一种动物基因组DNA提取方法。该方法具有简便、快速、实用的特点 ,所获得的DNA数量和质量都很高 ,可用于各种分子生物学实验。A very simple,fast method for extracting high quality genomic DNA from different tissues of animal is described.The method does not require expensive and environmentally hazardous reagents and equipment.The amount of tissue required by this method is about 50?mg.The quantity and quality of the DNA extracted by this method is high enough for performing thousands of PCR\|based reactions of other DNA manipulation such as restriction digestion,Southern blot and cloning.

关 键 词：动物组织 基因组DNA 快速提取 

分 类 号：Q95-3[生物学—动物学]