七宝美髯丹对60岁以上肝肾不足老年人黑白头发mtDNA异质性影响  

Influence of Qibao Meirandan on Black and White Hair mtDNA Heterogeneity of Over 60 Years Old People with Liver and Kidney Deficiency

作　　者：罗文峰[1] 程喜平[2] 谭凤明[2] 陈盛强[3] 

机构地区：[1]佛山市中医院皮肤科,广东佛山528000 [2]广州医学院第一附属医院皮肤科,广东广州510120 [3]广州医学院第二附属医院神经科学研究所,广东广州510160

出　　处：《辽宁中医药大学学报》2014年第2期19-21,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：佛山市医学类科技攻关项目(201108041)

摘　　要：目的 :研究七宝美髯丹对60岁以上肝肾不足老年人黑白头发mtDNA异质性影响。方法 :选择mtDNA高突变位点的编码区和控制区的2片段进行PCR扩增,以DHPLC技术分析黑、白头发mtDNA目标片段在七宝美髯丹治疗组和对照组异质性差异。结果 :七宝美髯丹治疗组黑头发在纳入观察时、纳入观察后30 d和60 d时mtDNA出现异质性比率和对照组比较均没有统计学差异;七宝美髯丹治疗组白头发在纳入观察后30 d和60 d时mtDNA出现异质性比率和对照组比较有下降,但没有统计学差异。以mtDNA编码区和控制区2个区域共60个mtDNA片段发生异质性总和数进行七宝美髯丹治疗组和对照组比较,纳入观察后60 d,二者有统计学差异。结论 :七宝美髯丹能减轻60岁以上肝肾不足老年人白头发mtDNA异质性。Objective： To study the influence of Qibao Meirandan on black and white hair mtDNA heterogeneity of the people aged over 60 with liver and kidney deficiency. Method： The tDNA from hair shaft was extracted, and PCR was used to amplify two target DNA fragments from mtDNA coding region and control region and analyze the heterogeneity differences in target DNA fragment between T2DM and control group with DHPLC. Results： There was no statistical significant difference in mtDNA heterogeneity ratio of black hair between Qibao Meirandan treatment group and control group at 0 0 day, 30 days or 60 days. The mtDNA heterogeneity ratio of white hair was decreased in Qibao Meirandan treatment group at 30 days and 60 days but there was also no statistical significant difference. There was statistical significant difference between Qibao Meirandan treatment group and control group at 60 days in heterogeneity ratio of 60 mtDNA fragments summarized by the mtDNA coding region and control region. Conclusion： Qibao Meirandan could reduce white hair mtDNA heterogeneity liver and kidney deficiency of people aged over 60 with liver and kidney deficiency.

关 键 词：头发 七宝美髯丹 MTDNA 异质性 变性高效液相色谱 

分 类 号：R289.5[医药卫生—方剂学]