蜜环菌多糖含药血清对Aβ_(25-35)诱导PC12细胞损伤保护机制  

Protective Mechanism of Armillaria Polysaccharide Serum on Injuries to PC12 Cell Induced by Amyloid β-Protein

作　　者：邱家权 殷银霞[2] 谢守嫔 李海龙 杨植媛 邓健男 吴玉泓[1] 

机构地区：[1]甘肃中医学院,甘肃兰州730000 [2]甘肃中医学院附属医院,甘肃兰州730000 [3]兰州市第一人民医院,甘肃兰州730050 [4]甘肃省中药新产品创制工程实验室,甘肃兰州730000

出　　处：《辽宁中医药大学学报》2014年第2期41-43,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：甘肃省中医药管理局资助项目(GZK-2010-47)

摘　　要：目的 :探讨蜜环菌多糖含药血清对Aβ25-35诱导大鼠肾上腺嗜铬细胞瘤细胞(PC12细胞)损伤保护的作用机制。方法 :在Aβ25-35诱导PC12细胞损伤模型的基础上加入蜜环菌多糖含药血清,采用比色法检测含药血清对Aβ25-35诱导PC12细胞损伤培养液中乳酸脱氢酶的活力、丙二醛含量及超氧化物歧化酶活力的影响。结果 :不同浓度的含药血清对Aβ25-35损伤的PC12细胞的存活率有提高作用,含药血清浓度在5%时达最大值(P<0.05或P<0.01);5%浓度的含药血清可明显降低模型细胞培养液中LDH活力、MDA含量,提高SOD活力。结论 :蜜环菌多糖含药血清对Aβ25-35损伤的PC12细胞有保护作用,机制可能与提高细胞抗氧化能力有关。Objective： To study the protective mechanism of armillaria polysaccharide serum on injuries of PC12 cells induced by Aβ25-35. Methods： Based on the model of injuries of PCI2 cells induced by Aβ25-35＇ the models were treated with armillaria polysaccharide serum at different concentrations. MTT method was used to detect the influence of armillaria polysaccharide serum to PC12 cell proliferation inhibited by Aβ25-35. Colorimetric method was used to detect the influence of armillaria polysaccharide serum on activity of lactic dehydrogenase, MDA content and SOD activity in damaged PC 12 cell culture. Results： Different concentrations of armillaria polysaccharide serum increased the survival rate of damaged PC 12 cells induced by Aβ25-35, the peak was at 5% （P〈0.05 or P〈0.01 ）; 5% concentration of armillaria polysaccharide serum can obviously reduce LDH activity, MDA content, and improve SOD activity in vitro. Conclusions： Armillaria polysaccharide can prevent PC 12 cell from Aβ25-35 injury, and the mechanisms of which may be related with the improved anti-oxidation ability of the cell.

关 键 词：阿尔茨海默病 蜜环菌多糖 AΒ25-35 PC12 

分 类 号：R931.71[医药卫生—生药学] R592[医药卫生—药学]