用PCR-SSO方法研究云南西部彝族HLA-DQB1基因的多态性  

Analysis of HLA-DQB1 Polymorphism by PCR-SSO in Yichu of Yunnan Province

作　　者：陈为民[1] 黄洪莲[1] 刘泽寰[1] 陶洪[2] 潘德京[1] 林蒋海[1] 徐安龙[1] 

机构地区：[1]中山大学生命科学学院,广州510275 [2]云南省卫生防疫站,昆明650000

出　　处：《Acta Genetica Sinica》2001年第2期107-114,共8页

基　　金：国家杰出青年基金!(编号:39725007)&&

摘　　要：应用PCR朣SO基因分型技术，对我国云南西部地区3代内无血缘关系的76个彝族健康个体进行了HLA-DQB1位点的基因分型。结果显示，在DQB1的38个等位基因中，观察到13个等位基因。云南西部彝族表现为DQB1*0301（36.18%～36.84%）最常见。其他频率大于5%的等位基因还有DQB1*0502（10.53%～11.18%）、DQB1*0401（9.21%）、DQB1*0302（8.55%～9.21%）、DQB1*0601（7.89%）、DQB1*05031（6.58%）、DQB1*03032（5.92%～6.58%）。和其他13个华人群体DQB1等位基因的频率比较分析表明，总体上，云南西部彝族和其他各华人群体间都存在很大的差异。显示其HLA等位基因频率分布的民族独特性。HLA-DQB1 genes from 76 individuals of Yichu ethnic group in Yunnan Province were investigated, using PCR-SSO genotyping method. Of the 38 DQB1 alleles detected, DQB10301 (gene frequency: 36.18%～36.84%) was the most common gene. The frequencies of DQB1*0502(10.53%～11.18%), DQB1*0401(9.21%), DQB1*0302(8.55%～9.21%), DQB1*0601(7.89%), DQB1*05031(6.58%), and DQB1*03032(5.92%～6.58%) are more than 5%. While DQB1*0504,DQB1*0604,DQB1*06052, DQB1*0606, DQB1*0607, DQB1*0608, DQB1*06112, DQB1*0613, DQB1*0615, DQB1*0203, DQB1*0305, DQB1*0306, DQB1*0307, and DQB1*0308 were not observed. Comparison of HLA-DQB1 allele frequencies of Yichu with those of 13 other Chinese ethnic groups showed some significant differences, suggesting Yichu is unique in the distribution of HLA alleles.

关 键 词：彝族 PCR-SSO LA-DQB1 云南 HLA基因 多态性 

分 类 号：Q987[生物学—遗传学]