水稻CMS-WA育性恢复基因的定位  

Mapping Genes for Rice CMS-WA Fertility Restoration

作　　者：庄杰云[1] 樊叶杨[1] 吴建利[1] 饶志明[1] 夏英武[2] 郑康乐[1] 

机构地区：[1]中国水稻研究所国家水稻改良中心,杭州310006 [2]浙江大学核农业科学研究所,杭州310029

出　　处：《Acta Genetica Sinica》2001年第2期129-134,共6页

基　　金：国家水稻基因组项目!(101-09-06);洛克菲勒基金会国际水稻生物技术项目!(RF99001#744)&&

摘　　要：在由227个组合组成的珍汕97A×（珍汕97B×密阳46）F6测交群体中，构建了由115个RFLP标记组成的连锁图。应用QTL分析方法，定位控制水稻CMS朩A（野败型细胞质雄性不育）育性恢复的基因。检测到1个主效基因qRf-10和3个效应较小的QTL（qRf-1、qRf-7和qRf-11），它们之间主要表现累加效应。同时，有证据表明这些基因之间存在相互作用。不仅微效基因qRf-1和qRf-11分别与主效基因qRf-10存在显著互作，qRf-10的存在与否，还影响到其他基因的相互作用，即可能存在多重互作。另外，还检测到2个控制结实率的QTL，它们不具有育性恢复作用，但在qRf-10存在的情况下，其杂合子具有提高结实率的作用。In a population consisting of 227 test-crosses of Zhenshan 97A×(Zhenshan 97B×Milyang 46)F6, a linkage map of 115 RFLP markers was constructed and employed to detect QTL for rice CMS-WA (wild abortive cytoplasmic male sterility) fertility restoration. One QTL having major effect, qRf-10, and 3 QTL having minor effects, qRf-1, qRf-7 and qRf-11, were detected. The four genes mainly acted additively with each other, but epistasis was also evident. Two minor QTL, qRf-1 andqRf-11, were significantly interacted with the major gene qRf-10. It was also found that interaction effects among minor QTL varied greatly depending on whether qRf-10 was present. Two other QTL, qSF-1 and qSF-7, were detected, which did not display any effects on fertility restoration. Instead, they showed effects for increasing SF when qRf-10 was present.

关 键 词：细胞质雄性不育 育性恢复基因 RFLP标记 水稻 基因定位 

分 类 号：Q943.2[生物学—植物学]