活性氧参与一氧化氮诱导的神经细胞凋亡  

Reactive Oxygen Species are Involved in Nitric Oxide-Induced Apoptosis of Neurons

作　　者：张春阳[1] 卫涛涛[2] 马辉[1] 丁尧[1] 陈瓞延[1] 侯京武[2] 陈畅[2] 忻文娟[2] 

机构地区：[1]清华大学物理系单原子分子测控教育部重点实验室,北京100084 [2]中国科学院生物物理研究所,北京100101

出　　处：《生物化学与生物物理进展》2001年第1期81-85,共5页Progress In Biochemistry and Biophysics

基　　金：清华大学 985重点项目;国家自然科学基金! (3980 0 0 35 )资助项目&&

摘　　要：采用激光共聚焦成像技术 ,用氧化还原敏感的特异性荧光探针 (DCFH DA和DHR12 3)直接研究了一氧化氮供体S 亚硝基 N 乙酰基青霉胺 (SNAP)诱导未成熟大鼠小脑颗粒神经元凋亡过程中的细胞胞浆、线粒体中活性氧水平的变化 ,发现神经细胞经 0 5mmol/LSNAP处理 1h后 ,细胞胞浆及线粒体中活性氧水平大大增加 .一氧化氮清除剂血红蛋白能够有效抑制细胞胞浆、线粒体中活性氧的产生 ,防止细胞凋亡 .外源性谷胱甘肽对细胞也具有良好的保护作用 ,而当细胞中谷胱甘肽的合成被抑制后 ,一氧化氮的神经毒性大大增强 .实验结果表明一氧化氮通过促进神经细胞产生内源性活性氧而启动细胞凋亡程序 ,而谷胱甘肽可能是重要的防止一氧化氮引发神经损伤的内源性抗氧化剂 .With redox sensitive fluorescene probes DCFH DA and DHR123, the formation of cytosolic and intramitochondrial reactive oxygen species (ROS) inside immature rat cerebellar granule cells during the apoptosis induced by nitric oxide donor S nitroso N acetyl pennicillamine (SNAP) was monitored by laser confocal scanning microscopy. The cytosolic and intramitochondrial ROS increase significantly after 0 5 mmol/L SNAP treatment for 1 h. Pre treatment with the nitric oxide scavenger hemoglobin can effectively inhibit the formation of cytosolic and intramitochondrial ROS and protect neurons from apoptosis. Adding glutathione can also protect neurons from apoptosis, and the cytotoxity of nitric oxide increases significantly while the synthesis of glutathione is inhibited. The results indicated that ROS might be involved in NO induced apoptosis in neural cells and glutathione might be the endogenesis antioxidant to protect neurons from oxidative injury.

关 键 词：小脑颗粒神经元 细胞凋亡 一氧化氮 活性氧 激光共聚焦成像 谷胱甘肽 

分 类 号：Q27[生物学—细胞生物学] Q42