用氨基修饰的载玻片制作cDNA微阵列  

Manufacture of Complementary DNA Arrays on Amino-modified Slides

作　　者：朱滨[1] 景奉香[1] 赵建龙[1] 孙悦[1] 陈继锋[1] 赵新泰[2] 徐元森[1] 

机构地区：[1]中国科学院上海冶金研究所 [2]上海肿瘤研究所,上海200030

出　　处：《生物化学与生物物理进展》2001年第1期121-124,共4页Progress In Biochemistry and Biophysics

基　　金：中国科学院九五特别支持项目! (KY95T 12 )&&

摘　　要：cDNA微阵列已在基因差异表达、寻找新基因等研究方面获得广泛应用 ,但有关cDNA微阵列的制作 ,目前多采用多聚赖氨酸修饰的载玻片为探针固定载体 ,固定效果较差 .用氨基硅烷处理的载玻片为载体制作cDNA微阵列 ,然后考察其固定效率、检测灵敏度、稳定性、实用性等指标 .结果表明 ,用氨基硅烷处理的载玻片具有比多聚赖氨酸更令人满意的核酸固定效率、检测灵敏度 ,且稳定实用 .因此 ,用氨基硅烷修饰的载玻片为探针固定载体制作cDNA微阵列较为理想 .The use of microarrays of oligonucleotides or cDNA is considered to be a promising approach for DNA and RNA sequence analysis, diagnostics of generic diseases, gene polymorphism studies and analysis of gene expression. To manufacture cDNA microarrays the samples were printed onto glass microscope slides treated with poly-L-lysine, and then the slides were processed by heat and UV light treatment to attach the cDNA sequence to the glass surface. But the immobilization efficiency of cDNA on the glass surface was low. A simple procedure for manufacture cDNA microarrays on a slide treated with 3-aminopropyltrimethoxysiiane is described. The efficiency for attaching cDNA to the amino-modified slides is greater than that to the slides treated with poly-L-iysine. The cDNA microarray made by the amino-modified slides is stable for use in 80 degreesC, 75% humidity, 3 600Lx light, exposure in air, respectively.

关 键 词：微阵列 生物芯片 修饰 CDNA 氨基修饰 载玻片 

分 类 号：Q523.03[生物学—生物化学]