根癌农杆菌对栝楼的遗传转化  

Genetic Transformation by Ti Plasmid in Trichosanthes kirilow ii

作　　者：雷和田[1] 宋经元[1] 祁建军[1] 张荫麟[1] 杨峻山[1] 郭志刚[2] 

机构地区：[1]中国医学科学院协和医科大学药用植物研究所,北京100094 [2]清华大学化学工程系,北京100084

出　　处：《云南植物研究》2001年第1期67-71,共5页Acta Botanica Yunnanica

摘　　要：用根癌农杆菌侵染栝楼(Trichosanthes kirilowii Maxim.) 无菌苗后,获得其冠瘿组织;栝楼冠瘿组织经除菌后能在无激素的MS培养基上良好生长,并合成冠瘿碱,表明Ti质粒转化成功。栝楼冠瘿组织中最高蛋白含量为130.6mg /g(鲜重)。经SDS-PAGE检测其含有的蛋白种类与栝楼根含有的基本一样。研究表明,利用栝楼冠瘿组织作为培养系统生产天花粉蛋白有很好的开发前景。Transformed crown tissue cultures from Trichosanthes kirilowii Maxim. were e stablished by Ti plasmid in C58 strains. The crown issues grew well in free- hor mone MS medium . Nopaline was inspected in the crown gall and this data showed t hat T-DNA of Ti plasmid had been transformed successfully .The content of protei ns in the tissue was about 130.6mg/g (fresh weight)which was inspected by compo und-color methods and its TCN-protein was inspected by SDS-PAGE analysis. The st udy showed that the production of TCN proteins from crown tissue cultures may be developed in prospect.

关 键 词：栝楼 Ti质粒转化 冠瘿组织培养 根癌农杆菌 遗传转化 

分 类 号：Q943[生物学—植物学] Q78