TLCS同时测定复方补乌糖浆中3种指标成分含量  

Simultaneous Determination of Three Marker Components in Fufang Buwu Syrup by TLCS

作　　者：陈莉[1,2] 王盛[1,2] 吴溪[1,2] 孟楣[1,2] 朱晶晶[1] 

机构地区：[1]安徽中医药大学第一附属医院,安徽合肥230031 [2]国家中医药管理局中药制剂三级实验室,安徽合肥230031

出　　处：《中国中医药信息杂志》2014年第2期92-94,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：安徽中医药大学青年科研基金(2012qn015)

摘　　要：目的建立同时测定复方补乌糖浆中3种成分含量的方法。方法采用薄层色谱扫描法（TLCS），硅胶G薄层板，环己烷-乙酸乙酯-二氯甲烷～甲酸（3：1：1：0．2）为展开剂，检测波长λs=300nm。结果阿魏酸在0．36～O．84gg、补骨脂素在0．12～0．28μg、大黄素在0．01～0．05μg线性范围内呈良好的线性关系，平均回收率分别为100．7％、100．8％、101．0％，RSD分别为1．26％、1．44％、1．86％。结论该方法简单、快速、高效，稳定可靠，可作为复方补乌糖浆的质量控制方法。Objective To establish an accurate method for determining the content of three components in Fufang Buwu Syrup. Methods TLC scanner was selected to detect three components with silica gel G thin layer plate. The sample was separated by using cyclohexane-ethyl acetate-methylenechloride-formic acid （3 ： 1 ： 1 ： 0.2）,λs=300 nm. Results The linearity between peak area and ferulie acid was achieved in the range of 0.36-0.84 μg, psoralen was achieved in the range of 0.12-0.28μg, emodin was achieved in the range of 0.01-0.05μg. The average recovery was 100.7%, 100.8%, 101.0%, and RSD was 1.26%, 1.44%, 1.86%, respectively. Conclusion The method is simple and accurate, which can be used for quality control of Fufang Buwu Syrup.

关 键 词：复方补乌糖浆 薄层色谱扫描法 含量测定 

分 类 号：R284.1[医药卫生—中药学]