Osterix在调控脊椎松质骨骨量中的作用  

The role of Osterix in controlling bone volume in vertebral body

作　　者：宗兆文[1] 陈思旭[1] 贾敏[1] 华祥[1] 郭庆山[1] 沈岳[1] 赵玉峰[1] 刘道城[1] Jerry Feng 

机构地区：[1]第三军医大学大坪医院野战外科研究所创伤/烧伤与复合伤国家重点实验室创伤科,重庆400042 [2]德克萨斯州卫生科学中心Baylor医学院生物医学系,美国德克萨斯州达拉斯75246

出　　处：《重庆医学》2014年第4期385-387,共3页Chongqing medicine

基　　金：创伤;烧伤与复合伤国家重点实验室自主课题(SKLZZ201124);"十二五"科研项目重大项目分课题(AWS11J008);国家自然基金面上项目(81271935)

摘　　要：目的观察Osterix在调控脊椎松质骨骨量的作用及机制。方法以Osterix敲除小鼠和Osterix转基因小鼠为观察对象,采用X线摄像、显微电子计算机X线断层摄影术(Micro-CT)和苏木素-伊红(HE)染色观察Osterix过表达或敲除后小鼠腰椎骨量的变化,抗酒石酸酸性磷酸酶(TRAP)染色观察破骨细胞的变化。免疫组织化学检测核因子-κB受体活化因子配体(RANKL)表达情况的变化。结果 Osterix转基因小鼠腰椎骨量没有明显变化,而X线片、Micro-CT和HE染色检测显示Osterix敲除小鼠在出生后12周脊椎密度和骨量增加;TRAP染色显示腰椎中破骨细胞数量减少;免疫组织化学染色检测显示其椎体中RANKL表达水平降低。结论 Osterix在调控脊椎松质骨骨量中起到重要作用。Objective To observe the role of Osterix in controlling bone volume in vertebral body and to investigate the possible mechanism .Methods X-ray radiology ,micro CT and HE staining were used to evaluate the change of bone volume in both Osterix knockout and transgenic mice .TRAP staining was used to assess the activity of osteoclasts and immunohistochemistry was used to examine the expression level of RANKL .Results No obvious changes were found in Osterix transgenic mice ,while X-ray examina-tion ,micro CT and HE staining showed that the bone density and bone volume in the lumbar vertebral body increased significantly in OSX null mice 12 weeks after birth .TRAP staining showed that the number of osteoclasts decreased in OSX null mice .IHC re-vealed that the expression level of RANKL was down-regualted in OSX null mice .Conclusion Osterix play an important role in controlling bone volume of vertebral body in mice .

关 键 词：脊柱 骨量 

分 类 号：R580[医药卫生—内分泌]