3种不同模式汇集白膜层法制备浓缩血小板质量和保存效果比较  

The comparison among the three models of pooled buffy coats in preparing the platelet concentrate

作　　者：王舒莹[1] 李晓明[2] 刘震岳[1] 张建民[1] 

机构地区：[1]承德医学院附属医院输血科,河北承德067000 [2]承德医学院附属医院检验科,河北承德067000

出　　处：《重庆医学》2014年第4期451-453,共3页Chongqing medicine

摘　　要：目的比较3种不同模式汇集白膜层(PBC)法制备浓缩血小板(PC)的质量及保存效果。方法使用3种不同模式PBC法制备PC,依据获得PC的模式不同分为即时PBC组、白膜法(BC)室温过夜组和全血(WB)室温过夜组,所有制备的PC过滤白细胞用血小板添加液(2/3PAS-ⅢM+1/3血浆)保存。比较3组PC在(22±2)℃保存7d内血小板含量、红细胞残留量、血小板聚集功能、CD62p阳性表达率、抗低渗休克(HSR)能力、pH值及细菌生长等指标。结果与即时PBC组相比,在保存期7d内BC室温过夜组血小板含量更高且能改善血小板聚集功能,而红细胞残留量、CD62p阳性表达率、HSR能力、pH值及细菌生长等指标无明显差异;与即时PBC组相比,在保存期7d内WB室温过夜组血小板含量更高且血小板HSR能力也更强,而红细胞残留量、血小板聚集功能、CD62p阳性表达率、pH值及细菌生长等指标两组差异无统计学意义(P>0.05)。结论 BC室温过夜、PBC法和WB室温过夜PBC法均能安全、可靠、方便地制备PC,且均可代替即时PBC法制备PC。Objective To compare the quality and preservation effect among the three models of pooled buffy coats （PBC） in pre-paring the platelet concentrate （PC） .Methods 63 identical ABO blood donations were randomly and averagely divided into three groups ：the immediate PBC group（n=21） ,the buffy coats（BC） group（n=21） and the whole blood（WB） group（n=21） .In the im-mediate PBC group ,both the separation of the BC and the preparation of the PC was finished in the first day after collecting the WB;in the BC group ,the separation of BC was executed in the first day after collecting the WB ,while the preparation of the PC was completed in the second day ;in the WB group ,both the separation of the BC and the preparation of the PC was implement in the second day after collecting the WB .All the prepared PC were storage at （22 ± 2） ℃ for seven days in the preservation solution （composed by 2/3 PAS-ⅢM and 1/3 plasma） .Then compare the platelet（PLT） counts ,the red blood cell（RBC） residual quanti-ties ,the aggregative function of PLT ,the positive expression rates of CD62p ,the capability of hypotonic shock response（HSR） ,the PH value and the bacterial growth among PCs prepared by the three models during the stored time .Results Compared the immedi-ate PBC group during the seven stored days ,the PLT counts of the BC and WB groups were more ,the aggregative function of PLT in the BC group was better and the capability of HSR in the WB group was stronger ,but the other indexs between the immediate PBC group and the BC or WB group were no significant difference（P〉0 .05） .Conclusion Both an overnight holding of BC and WB at room temperature models of PBC to prepare the PC are safe ,reliable and convenient ,and they could be substitute for the immedi-ate PBC model to prepare the PC .

关 键 词：浓缩血小板 汇集白膜层法 质量分析 

分 类 号：R457[医药卫生—治疗学]