圈卷产色链霉菌尼可霉素生物合成基因—sanL的结构与功能  

STRUCTURE AND FUNCTION OF sanL GENE INVOLVED IN NIKKOMYCIN BIOSYNTHESIS OF STREPTOMYCES ANSOCHROMOGENES

作　　者：聂丽平[1,2] 张集慧[1] 谭华荣[1] 

机构地区：[1]中国科学院微生物研究所 [2]辽宁师范大学生物系大连116029

出　　处：《微生物学报》2001年第1期59-64,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金!重点项目 ( 39830 0 1 0 )资助;微生物资源前期开发国家重点实验室支持&&

摘　　要：利用染色体步移策略 ,以尼可霉素生物合成相关的基因片段为探针 ,从圈卷产色链霉菌中克隆到了一个大约 1 0kb的DNA片段。序列分析表明此片段中除含有sanK外 ,在sanK的上游还有一个完整开放阅读框———sanL。sanL与sanK的转录方向相同 ,具有 1 2 81个核苷酸 ,起始密码子为 345位的ATG ,终止密码子为 1 62 3位的TGA。利用Blastx程序进行的分析揭示 ,此基因可能编码一个赖氨酸 2 氨基转移酶。基因功能研究表明 ,该基因是圈卷产色链霉菌尼可霉素生物合成所必需的。The chromosome walking strategy has been used in this experiment and a 10kb DNA fragment was cloned from the cosmid library of Streptomyces ansochromogenes using a partial DNA fragment involved in the nikkomycin biosynthesis as a probe.The nucleotide sequence showed that the DNA fragment contains two complete open reading frames (ORFs),they are designated as sanK and sanL respectively.The \%sanL\% is located on the upstream of \%sanK\%,it has 1281 nucleotides with ATG at 345 position as start codon and TGA at 623 position as stop codon.The deduced product is L\|lysine 2\|aminotransferase using Blastx program.The experiment of gene disruption indicated that this gene is closely related to nikkomycin biosynthesis of Streptomyces ansochromogenes.

关 键 词：圈卷产色链霉菌 尼可霉素 生物合成 赖氨酸-2-氨基转移酶 抗生素 

分 类 号：Q75[生物学—分子生物学]