肥胖对大鼠前列腺炎性反应的影响及其相关机制  

The research of influence and related mechanism of obesity on prostatitis in rats

作　　者：刘世学[1] 李可[1] 谢斌[1] 张厚彬 

机构地区：[1]重庆市巴南区人民医院泌尿外科,401320

出　　处：《重庆医学》2014年第4期469-470,473,共3页Chongqing medicine

基　　金：重庆市巴南区卫生局课题(2013-01-16)

摘　　要：目的通过高脂饲料诱发的SD大鼠肥胖模型研究肥胖对慢性前列腺炎(CP)发病的影响及其相关机制。方法实验分为普通饲料组、高脂饲料组、阳性对照组。通过喂养高脂饲料建立大鼠肥胖模型;前列腺液镜检检测各组大鼠卵磷脂小体密度和白细胞计数;采用ELISA检测前列腺液中炎症因子IL-6和IL-8的分泌;实时定量-聚合酶链反应(RT-PCR)检测炎症相关基因IL-6和IL-8的表达水平。结果高脂饲料喂养大鼠5周后出现明显肥胖;高脂饲料组大鼠前列腺炎发病率明显高于普通饲料组,其前列腺液中IL-6和IL-8的分泌明显高于普通饲料组;高脂饲料组前列腺组织IL-6和IL-8的基因表达也明显升高。结论肥胖能够明显升高大鼠的前列腺组织炎症因子IL-6和IL-8的分泌和基因表达,从而导致CP发病率升高。Objective To investigate the effects of high fat diet induced obesity on rat prostatitis and assesses the metabolic fea-tures .Methods The normal diet group ,high-fat diet group and the positive control group were established according to the experi-mental requirements .Obesity model was established due to high-fat-diet feed .Microscopy was performed to analyze Lecithin density and leucocyte count .IL-6 and IL-8 Release level in the prostatic fluid were measured with ELISA （enzyme-linked immunosorbent as-say） kits according to the manufacturer′s directions .IL-6 and IL-8 mRNA levels of prostate were measured by real time PCR .Re-sults Compared with normal diet group ,high-fat diet group tended to be obesity obviously in 5 weeks of high-fat diet .The inci-dence rate of prostatitis in high fat diet group was significantly higher than that of control group ,and high-fat diet group had higher IL-6 and IL-8 releasing and mRNA levels .Conclusion High-fat diet could increase incidence of prostatitis in rat .The effect is par-tially due to IL-6 and IL-8 releasing and expression .

关 键 词：大鼠 前列腺炎 肥胖 炎症因子 

分 类 号：R697.33[医药卫生—泌尿科学]