微孔板杂交法测定椰毒假单胞酵米面亚种的DNA-DNA同源性  

DETERMINATION OF DNA-DNA HOMOLOGY AMONG PSEUDOMONAS COCOVENENANS SUBSP. FARINOFERMENTANS WITH MICRODILUTION PLATE HYBRIDIZATION METHOD

作　　者：焦振泉[1] 刘秀梅[1] 杨瑞馥[2] 孟昭赫[1] 

机构地区：[1]中国预防医学科学院营养与食品卫生研究所,北京100050 [2]中国军事医学科学院微生物与流行病研究所,北京100071

出　　处：《微生物学报》2001年第1期70-75,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金资助项目!( 39570 61 9)&&

摘　　要：采用一种新的DNA DNA杂交方法—微孔板法对椰毒假单胞菌酵米面亚种及伯克霍尔德氏菌属中 1 1个种的DNA DNA同源性进行测定。结果发现椰毒假单胞菌酵米面亚种与唐菖蒲伯克霍尔德氏菌及椰毒伯克霍尔德氏菌的DNA DNA同源性均大于 75% ,建议这 3个种应合并为同一个种 ,命名为唐菖蒲伯克霍尔德氏菌 (Burkholderiagladioli)。The DNA\|DNA homologies among Pseudomonas cocovenenans subsp. farinofermentans and 11species in genus Burkholderia were determined with microdilution plate hybridization method. The results showed:the homologies among P.cocovenenans subsp. farinofermentans,B.gladioli and B.cocovenenans were all over75%.It was advised that these three species were synonym,and should be renamed as Burkholderia gladioli.

关 键 词：郴毒假单胞酵米面亚种 DNA-DNA同源性 微孔板法 

分 类 号：Q939.09[生物学—微生物学]