水稻半矮秆基因sd-t的染色体定位研究  

Chromosome Location of a Semidwarf Gene sd-t in indica Rice (O.sativa L.)

作　　者：李欣[1] 顾铭洪[1] 梁国华[1] 徐金凤[1] 陈宗祥[1] 杨海生[1] 

机构地区：[1]扬州大学农学院农学系,扬州225009

出　　处：《Acta Genetica Sinica》2001年第1期33-40,共8页

基　　金：国家自然科学基金!(39470443)

摘　　要：以籼型标志基因系和ＩＲ３６三体为工具材料，通过杂交研究了籼稻矮秆材料矮泰引－２所携半矮秆基因Ｓｄ－ｔ在染色体上的位置。结果表明，半矮秆基因Ｓｄ－ｔ与标志基因系０１９所携紫果皮基因Ｐｒｐ－ｂ、标志基因系Ｂ３０所携无叶舌基因１ｇ、标志基因系０２７所携灰白壳基因Ｗｈ表现连锁，ｓｄ－ｔ与Ｐｒｐ－ｂ之间的交换值为２．８５％±０．５２％，ｓｄ－ｔ与ｌｇ之间的交换值为２７．９０％±３．８１％，ｓｄ－ｔ与Ｗｈ之间的交换值为３８．６２％±２．９９％。由于Ｐｒｐ－ｂ、ｌｇ、Ｗｈ基因均位于第４染色体上，因而推定ｓｄ－ｔ基因位于第４染色体上，其排列位置可能是Ｐｒｐ－ｂ－ｓｄ－ｔ－ｌｇ－Ｗｈ。For investigating the linkage group to which the semidwarf gene sd-t belongs, the crosses between Aitaiyin 2(ATY2), a variety of sd-t carrier in indica rice, and marker stocks as well as the trisomic lines of IR36 were made. The results indicated that the sd-t gene was linked to the marker genes Prp-b (Purple pericap b). lg(ligueless) and Wh(White hull). The recombination values estimated were 2.85% ± 0.52% for sd-t to Prp-b. 27.90% ± 3.81% for sd-t to lg and 38.62% ± 2.99% for sd-t to Wh, respectively. It was inferred that the sd-t gene was localed on chromosome 4 according to the established relationship between chromosomes and linkage groups.

关 键 词：水稻 半矮秆基因 标志基因 三体 染色体定位 

分 类 号：Q943.2[生物学—植物学]