量子点标记天花粉蛋白的研究  

Studies on Quantum Dots-labeled Trichosanthin

作　　者：张春阳[1] 马辉[1] 丁尧[1] 金雷[1] 陈瓞延[1] 苗琦[2] 聂书明[3] 

机构地区：[1]清华大学物理系单原子分子测控教育部重点实验室,北京100084 [2]中国科学院生物物理研究所生物大分子国家重点实验室,北京100101 [3]印第安那大学化学系

出　　处：《高等学校化学学报》2001年第1期34-37,共4页Chemical Journal of Chinese Universities

基　　金：国家基金委海外青年学者合作基金!(批准号 :19992 840 8);清华大学985基金项目资助

摘　　要：将半导体量子点应用于标记天花粉蛋白 ,研究了量子点标记天花粉蛋白的吸收光谱、荧光光谱和酶活性变化 .与游离的 QDs相比 ,QDs-TCS的吸收光谱在 4 0 0～ 60 0 nm范围内不发生明显变化 ;QDs-TCS的发射光谱发生蓝移 ,但发射半宽不变 ;标记上 QDs后 TCS的酶活性没有发生改变 .利用双光子激发扫描荧光显微镜和激光共聚焦显微镜同时观察 QDs-TCS在人绒癌细胞内的分布发现 ,QDs-TCS能够跨膜进入细胞 ,并在细胞核周围呈聚集分布 .Semiconductor quantum dots were used for the first time to label trichosanthin. The absorption spectrum, fluorescent spectrum and enzymatic activity of quantum dots-labeled trichosanthin were studied in this paper. The absorbane of QDs-TCS varied to a less extent with wavelength in the range of 400\_600 nm. The fluorescent spectrum of QDs-TCS underwent a blue-shift in the emission peak, but the intrinsic spectral width was unchanged. No changes were found in the enzymatic activity of QDs-TCS in comparison with trichosanthin. Distribution of QDs-TCS within human choriocarcinoma cells was simultaneously observed with two-photon laser scanning microscopy and confocal laser scanning microscopy. The results indicated that QDs-TCS could enter the cytoplasm across the membrane and aggregated around the nuleolus.

关 键 词：天花粉蛋白 酶活性 半导体量子点 标记 中药研究 生物荧光探针 

分 类 号：Q5[生物学—生物化学] R284[医药卫生—中药学]