钩藤生物碱中一对异构体的TLC-FT-SERS研究  

TLC-FT-SERS Study on a Pair Isomer in Uncaria Rhynchophylla Jacks Alkali

作　　者：汪瑗[1] 王松颖[2] 赵宜学[3] 訾凤兰[1] 王英峰[1] 汪洪杰[1] 张敬冰[1] 

机构地区：[1]首都师范大学化学系,北京100037 [2]北京理工大学物理系,北京100081 [3]北京教育学院化学系,北京100044

出　　处：《高等学校化学学报》2001年第1期46-48,共3页Chemical Journal of Chinese Universities

基　　金：北京中关村地区联合测试中心测试基金;首都师范大学重点科研项目资助

摘　　要：A new method for analysing the ingredients of rhynchophylline and isrhynchophylline in Uncaria Rhynchophylla Jacks using hyphenaed high efficiency thin layer chromatographic（TLC） and the surface-enhanced raman spectroscopic（SERS） technics is reported. The results show that the characteristic spectral bands of rhynchophylline can be obtained from the TLC spot with minimum amount of rhynchophylline sample of about 2 5 mm diameter. The difference between the SERS and solid spectra are found. Spectral band at 1 397 cm-1 was obtained wits great enhanced want. Molecule was absorbed in surface silver sol by pair of electrons in —OCH3. Isrhynchophylline sample spectrum band at 1 615 cm-1 obtained the greatest enhance. Isrhynchophylline molecule was absorbed in surface silver sol by pair of electron at N atom in ring indole. An absorption model of rhynchophylline and isrhynchophylline and silver sol is proposed. That effective combination of TLC and SERS can be used to analyse the chemical ingredients with high sensitivity.A new method for analysing the ingredients of rhynchophylline and isrhynchophylline in Uncaria Rhynchophylla Jacks using hyphenaed high efficiency thin layer chromatographic(TLC) and the surface-enhanced raman spectroscopic(SERS) technics is reported. The results show that the characteristic spectral bands of rhynchophylline can be obtained from the TLC spot with minimum amount of rhynchophylline sample of about 2 5 mm diameter. The difference between the SERS and solid spectra are found. Spectral band at 1 397 cm^-1 was obtained wits great enhanced want. Molecule was absorbed in surface silver sol by pair of electrons in —OCH_3. Isrhynchophylline sample spectrum band at 1 615 cm^-1 obtained the greatest enhance. Isrhynchophylline molecule was absorbed in surface silver sol by pair of electron at N atom in ring indole. An absorption model of rhynchophylline and isrhynchophylline and silver sol is proposed. That effective combination of TLC and SERS can be used to analyse the chemical ingredients with high sensitivity.

关 键 词：钩藤碱 异钩藤碱 异构体 薄层色 表面增强喇曼散射 分离 检测 天然药物 生物碱 钩藤 

分 类 号：R284.1[医药卫生—中药学]