巴西橡胶树橡胶粒子中2个含RicinB_Lectin_2结构域蛋白质研究初报  

A Preliminary Report on Two Rubber Particles Proteins Containing RicinB_Lectin_2 Domain from Hevea brasiliensis

作　　者：代龙军[1] 黎瑜[1] 康桂娟[1] 聂智毅[1] 段翠芳[1] 曾日中[1] 

机构地区：[1]中国热带农业科学院橡胶研究所/农业部橡胶树生物学与遗传资源利用重点实验室/省部共建国家重点实验室培育基地,海南儋州571737

出　　处：《热带农业科学》2014年第1期48-53,共6页Chinese Journal of Tropical Agriculture

基　　金：国家自然科学基金(No.31270713);海南省自然科学基金(No.312028);中国热带农业科学院橡胶研究所基本科研业务费专项(No.1630022013014)

摘　　要：采用不同提取液,从洗涤过的巴西橡胶树橡胶粒子中提取蛋白质,并对其进行电泳分离与质谱鉴定。分离鉴定了2个含RicinB_Lectin_2结构域的糖基识别蛋白质,分别命名为HbRBLLP-1和HbRBLLP-2。这2个蛋白质可能是橡胶粒子膜蛋白质或是与橡胶粒子有较强的相互作用的蛋白质。对HbRBLLP-1及HbRBLLP-2进行生物信息学分析,结果表明它们的RicinB_Lectin_2结构域均仅有1个Q-x-W模体,而非蓖麻毒素B链所具有的(Q-x-W)3模体。Proteins extracted with different buffers from washed Hevea brasiliensis rubber particles were subjected to electrophoresis and mass spectrometry analysis. Two carbohydrate binding RicinB_Lectin_2 domain containing proteins were isolated and identified, and these two proteins were designated as HbRBLLP-1 and HbRBLLP-2, respectively. These two proteins may be membrane proteins of rubber particles or proteins have a strong interaction with rubber particles. Bioinformatics analysis was also carried out on these two proteins. Bioinformatics analysis showed that HbRBLLP-1 and HbRBLLP-2 have only one QxW motif in their RicinB_Lectin_2 domain, respectively; whereas, ricin B chain has a （QxW）3 motif.

关 键 词：巴西橡胶树 橡胶粒子 蛋白质 RicinB_Lectin_2结构域 

分 类 号：S794.1[农业科学—林木遗传育种]