应用311-A最优回归设计研究ABA、PEG4000及AgNO_3对落叶松体细胞胚发生数量的影响  

Study on Effect of ABA, PEG4000 and AgNO_3 on Number of Somatic Embryos of Larix Principis-rupprechtii by 311-A Regression Design

作　　者：齐力旺[1] 韩一凡[1] 李玲[1] Dietrich Ewald 韩素英[3] 

机构地区：[1]中国林科院林业研究所分子遗传室,北京100091 [2]Federal Research Centre for Forestry Institute for Forestry Tree Breeding and Biotechnology,EberswalderChaussee6 [3]山西农业大学林木生物技术研究中心,太谷030801

出　　处：《生物工程学报》2001年第1期84-89,共6页Chinese Journal of Biotechnology

基　　金：国家自然科学基金!(39870 6 32 );国家林业局"948"项目!(98 4 0 4 0 2 )资助&&

摘　　要：采用三因素多项式回归 311 A最优设计方法对影响落叶松体细胞胚发生数量的ABA、PEG40 0 0和AgNO3的用量进行了研究 ,建立了华北落叶松正常体细胞胚发生数量与ABA、PEG40 0 0和AgNO3 的数学模型 ,分析了落叶松体细胞胚发生数量试验因子的主效应和互作效应 ,优选了落叶松体细胞胚发生数量最佳结果的最佳技术组合方案为ABA18 9138mg/L ,PEG40 0 0 88 80 0 7g/L与AgNO310 .75 13mg/L时 ,最佳体细胞胚数量应为 10 7 5 2 78个子叶胚 /g .callus。实验结果表明 :该方法是针叶树体细胞胚胎发生过程中 ,主要激素种类与浓度配比、处理组合 ,科学、合理的培养基优化途径。This paper, the effects of ABA, PEG400 and AgNO\-3 on the number of somatic embryos of \%Larix Principis\|Rupprechtii\% were carried out by 311\|A regression design. The regression equation was established which expresses the number of somatic embryos of \%Larix Principis\|Rupprechtii\% as function, and the ABA, PEG400 and AgNO\-3 on as variable respectively. Using the regression equation, the single factor effect and mutual effect between the number of somatic embryos of \%Larix Principis\|Rupprechtii\% and ABA, PEG400 and AgNO\-3 was studied; the optimum concentration recipes can be obtained by computer processing, that are ABA: 18.9138mg/L, PEG400:88.8007g/L and AgNO\-3:10 7513mg/L, the target somatic embryos number is 107.5278 each gram callus. The results of experiment showed that this method is simple, practical and rapid for selecting several hormone category and concentration recipes media of conifer somatic embryogenesis.

关 键 词：华北落叶松 311-A设计 体细胞胚发生数量 主效 

分 类 号：Q943[生物学—植物学]