淡水白鲳细胞建系及其生长温度特性的研究  

The Establishment of Four Cell Lines from Colossoma brachypomum and Their Growth Characteristics Under Various Temperatures

作　　者：张铭[1] 陈荪红[2] 赵小立[1] 

机构地区：[1]浙江大学生命科学学院,杭州310012 [2]上海第二医科大学,上海200025

出　　处：《生物工程学报》2001年第1期105-108,共4页Chinese Journal of Biotechnology

基　　金：浙江省自然科学基金资助项目(394339)

摘　　要：Four cell lines originated from embryo,caudal fin,anal fin and snout of Colossoma brachypomum were established,and subcultured for 70,47,45 and 40 passages to the present.The results of the experiment indicated that the four cell lines were fibroblast\|like diploid cells,and the model number of their chromosome is 54.These cell lines could grow under temperature of 20-35℃,and its optimum temperature was 27-30℃.The cells grew slowly at 20℃,stopped growth at 15℃,and died below 10℃.This indicated that the growth temperature of the these cell lines was coincident with that of fish.In addition,the attachment time,doubling time,mitotic index,passage survival rate and cell cycle were determined and compared.Four cell lines originated from embryo,caudal fin,anal fin and snout of Colossoma brachypomum were established,and subcultured for 70,47,45 and 40 passages to the present.The results of the experiment indicated that the four cell lines were fibroblast\|like diploid cells,and the model number of their chromosome is 54.These cell lines could grow under temperature of 20-35℃,and its optimum temperature was 27-30℃.The cells grew slowly at 20℃,stopped growth at 15℃,and died below 10℃.This indicated that the growth temperature of the these cell lines was coincident with that of fish.In addition,the attachment time,doubling time,mitotic index,passage survival rate and cell cycle were determined and compared.

关 键 词：淡水白鲳 细胞系 胚胎 动物细胞培养 

分 类 号：Q954.633[生物学—动物学]