α7尼古丁受体介导100Hz电针调节自然杀伤细胞活性的实验研究  

New evidences of α7nAChR participating in 100 Hz EA regulating NK cells activity

作　　者：胡志苹[1] 黄志华[1] 黄诚[1] 

机构地区：[1]赣南医学院基础医学院,江西赣州341000

出　　处：《重庆医学》2014年第5期582-583,586,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(31060144);江西省自然科学基金资助项目(2008GZY0067)

摘　　要：目的在已有的实验基础上,进一步寻求α7尼古丁受体(α7nAChR)介导100Hz电针(EA)调节自然杀伤细胞(NK)细胞活性的新证据。方法应用α7nAChR抗体和特异性的α7nAChR拮抗剂α-银环蛇毒(α-Bgt),探讨α7nAChR在EA对NK细胞活性中的作用。结果与100Hz EA组相比,100Hz EA+α7nAChR抗体组脾脏NK细胞活性明显增强,差异有统计学意义(P<0.05);与100Hz EA组相比,1.5和3.0μgα-Bgt+100Hz EA组均NK细胞活性明显增强,差异有统计学意义(P<0.01)。结论α7nAChR介导了100Hz EA调节免疫功能的作用,这为进一步明确和扩展EA的临床应用提供理论和实践依据。Objective On the basis of previous experiments ,we try further to seek the new evidences of α7nAChR participating in 100 Hz electroacupuncture （EA ） regulating natural killer （NK ） cells activity .Methods α7nAChR antibody and the specificα7nAChR antagonist α-bungarotoxin （α-Bgt） were used to study the effect of α7nAChR on NK cell activity in 100 Hz EA mice .Re-sults Compared with 100 Hz EA group ,100 Hz EA plus α7nAChR-Ab group showed a significant enhanced in NK cell activity （P〈0 .05） .100 Hz EA treated with 1 .5 or 3 .0 μg α-Bgt groups had higher NK cell activity than 100 Hz EA group（P〈0 .01） . Conclusion α7nAChR is involved in 100 Hz EA modulation for immune function ,which provide the theoretical and practical basis for further clarifying and expanding the clinical application of EA .

关 键 词：电针 自然杀伤细胞活性 α7尼古丁受体 

分 类 号：R245[医药卫生—针灸推拿学]