细胞外钙调素对百合花粉细胞内钙离子浓度的影响  

Effect of Extracellular Calmodulin on the Cytosolic Ca^(2+) Concentration in Lily Pollen Grains

作　　者：尚忠林[1] 马力耕[1] 王学臣[2] 孙大业[1] 

机构地区：[1]河北师范大学生命科学学院,石家庄050016 [2]农业部植物生理生化重点开放实验室,北京100094

出　　处：《Acta Botanica Sinica》2001年第1期12-17,共6页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家和河北省自然科学基金重点!( 3 993 0 2 3 0 )及国家重点基础研究规划项目! (G19990 1170 0 )&&

摘　　要：以川百合 (LiliumdavidiiDuchartre)花粉为材料 ,利用低温装载法在完整花粉粒中成功地装载了酯化形式的钙离子荧光指示剂fluo_3AM ,利用激光共聚焦显微技术研究了细胞外钙调素对细胞内游离钙离子浓度的影响。结果发现外源纯化钙调素可以使细胞内游离钙离子的浓度升高 ,在一定范围内促进效果与钙调素浓度呈正相关。不能透过细胞膜的钙调素拮抗剂W7_agarose和植物钙调素抗血清处理都可以使花粉细胞质中游离钙离子浓度降低 。Using Lilium davidii Duchartre pollen as material, the calcium ion_fluorescence indicator fluo_3AM was loaded successfully into the pollen grains by low temperature loading method. Laser confocal scanning microscopy was used to study the effect of extracellular calmodulin on intracellular calcium. It is found that the purified exogenous calmodulin could elevate the intracellular calcium ion concentration, and the effect was correlated with the concentration of exogenous calmodulin to a certain extent. Cell membrane nonpermeable inhibitor of calmodulin, W 7_agarose, and the anti_serum of calmodulin could decrease the cytosolic calcium level. The results show that the endogenous extracellular calmodulin may play an important role in maintaining and increasing the cytosolic calcium level in pollen grain cell.

关 键 词：川百合 花粉 细胞外钙调素 游离钙离子 低温装载 激光共聚焦显微术 植物发育生理 

分 类 号：Q942[生物学—植物学]