Agrobacterium _mediated Transformation of Rice with Help of Bombardment  

通过基因枪提高根癌土壤杆菌转化水稻的效率(英文)

作　　者：明小天[1] 苑华毅[1] 王莉江[1] 陈章良[1] 

机构地区：[1]北京大学生命科学学院蛋白质工程和植物基因工程国家重点实验室,北京100871

出　　处：《Acta Botanica Sinica》2001年第1期72-76,共5页Acta Botanica Sinica（植物学报：英文版）

摘　　要：Oryza sativa L. ssp. japonica cv. Zhonghua 8, which is recalcitrant to infection of Agrobacterium tumefaciens (Smith et Townsend) Conn strain EHA105 with ordinary binary vector pCambia 1301, was transformed through Agrobacterium-mediated transformation with help of bombardment. The transformation efficiency can be raised greatly. Single copy of gene insertion in the genome of transgenic rice plants was proved by Southern analysis and the expression of GUS gene was observed. GUS gene and hygromycin-resistant gene show 3:1 segregation in progenies of the transgenic rice plants.携带普通双元载体的根癌土壤杆菌 (Agrobacteriumtumefaciens (SmithetTownsend)Conn)EHA10 5菌株对粳稻(OryzasativaL .ssp .japonica)中花 8号不敏感 ,转化效率较低。利用基因枪将根癌土壤杆菌细胞直接轰击到水稻愈伤组织中可以显著提高其转化效率。Southern检测证明转基因植物含有单拷贝的外源基因插入片段。转基因植物后代的GUS基因表达呈

关 键 词：AGROBACTERIUM BOMBARDMENT TRANSFORMATION RICE 

分 类 号：Q943.2[生物学—植物学]