Observation of cavitation and water-refilling processes in plants with X-ray phase contrast microscopy  

作　　者：XUE Yanling XIAO Tiqiao DU Guoha TONG Yajun LIU Huiqiang DENG Biao XIE Honglan XU Hongjie 

机构地区：[1]Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Zhangjiang Campus

出　　处：《Nuclear Science and Techniques》2013年第6期1-6,共6页核技术（英文）

基　　金：Supported by the National Natural Science Foundation of China(No.11105213);the State Key Development Program for Basic Research of China(No.2010CB834301);the External Cooperation Program of the Chinese Academy of Sciences(No.GJHZ09058);the Knowledge Innovation Program of the Chinese Academy of Sciences

摘　　要：With the spatial coherence of X-rays and high flux and brightness of the 3rd generation synchrotron radiation facility,X-ray phase contrast microscopy(XPCM)at Shanghai Synchrotron Radiation Facility(SSRF)can provide high resolution dynamic imaging of low electron density materials in principle.In this paper,we investigated the cavitation and water-refilling processes in rice and bamboo leaves utilizing XPCM at SSRF.The occurrence of xylem cavitation was recorded in vivo.The study also revealed that under different dehydration conditions,cavitation occurs in different degrees,and therefore,the refilling process is different.The results demonstrate that SSRF can provide high enough fluxes to study dynamic processes in plants in real-time,and XPCM is expected to be a promising method to reveal the mechanisms of cavitation and its repair in plants nondestructively.With the spatial coherence of X-rays and high flux and brightness of the 3rd generation synchrotron radiation facility, X-ray phase contrast microscopy （XPCM） at Shanghai Synchrotron Radiation Facility （SSRF） can provide high resolution dynamic imaging of low electron density materials in principle. In this paper, we investigated the cavitation and water-refilling processes in rice and bamboo leaves utilizing XPCM at SSRF. The occurrence of xylem cavitation was recorded in vivo. The study also revealed that under different dehydration conditions, cavitation occurs in different degrees, and therefore, the refilling process is different. The results demonstrate that SSRF can provide high enough fluxes to study dynamic processes in plants in real-time, and XPCM is expected to be a promising method to reveal the mechanisms of cavitation and its repair in plants nondestructively.

关 键 词：相衬显微镜 显微镜观察 植物 X-射线 填充过程 气蚀 上海同步辐射装置 水稻 

分 类 号：Q945[生物学—植物学]