Risk of simulated microgravity on testicular injury induced by high-LET carbon-ion beams in mice  

作　　者：LIU Yang ZHANG Luwei ZHANG Hong WU Zhenhua Wang Zhenhua WANG Yibo LI Hongyan MA Xiaofei XIE Yi 

机构地区：[1]Department of Heavy Ion Radiation Medicine,Institute of Modern physics,Chinese Academy of Sciences [2]Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences [3]Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province [4]College of Life Science and chemistry,Tianshui Normal University

出　　处：《Nuclear Science and Techniques》2013年第6期61-66,共6页核技术（英文）

基　　金：Supported by grants from the National Basic Research Program of China(No.2010CB834202);Knowledge Innovation Projection of Chinese Academy of Science(No.KJCX2-YW-L08);The National Natural Science Foundation of China(No.10835011,No.10675151,No.41161058,No.31060065 and No.10805064)

摘　　要：This study investigated the impact of simulated microgravity on acute injury induced by low doses of carbon ions in male reproductive organs of mice,and determined alterations in spermatogenic function and expression levels of apoptotic factors in mice following exposure to acute irradiation after 7 days of simulated microgravity.The results demonstrated that significant reductions in spermatozoa,primary spermatocytes and spermatogonia,and increased globular cells in seminiferous tubule and pro-apoptotic proteins were observed in the group exposed to over0.4 Gy irradiation.Collectively,the data suggest that lesions inflicted by simulated microgravity are not markedly modified by lower doses of irradiation(0.2 Gy)in mouse testis compared to the control group.However,testicular impairments were markedly evident in the group exposed to higher doses of carbon ions plus simulated microgravity,which may be due at least in part to elevated apoptosis initiated by the mitochondrial apoptosis pathway in germ cells.This study investigated the impact of simulated microgravity on acute injury induced by low doses of carbon ions in male reproductive organs of mice, and determined alterations in spermatogenic function and expression levels of apoptotic factors in mice following exposure to acute irradiation after 7 days of simulated microgravity. The results demonstrated that significant reductions in spermatozoa, primary spermatocytes and spermatogonia, and increased globular cells in seminiferous tubule and pro-apoptotie proteins were observed in the group exposed to over 0.4 Gy irradiation. Collectively, the data suggest that lesions inflicted by simulated microgravity are not markedly modified by lower doses of irradiation （0.2 Gy） in mouse testis compared to the control group. However, testicular impairments were markedly evident in the group exposed to higher doses of carbon ions plus simulated microgravity, which may be due at least in part to elevated apoptosis initiated by the mitochondrial apoptosis pathway in germ cells.

关 键 词：模拟微重力 小鼠睾丸 碳离子束 损伤 精原干细胞 风险 诱导 雄性生殖器官 

分 类 号：Q492.4[生物学—生理学]