环介导等温扩增技术在病原菌检测中的应用  

Loop-Mediated Isothermal Amplifi cation Method and Its Application in Pathogen Inspection

作　　者：刘芳[1] 黄静敏[1] 何永盛[1] 李传礼[1] 

机构地区：[1]深圳市计量质量检测研究院,广东深圳518131

出　　处：《检验检疫学刊》2013年第6期68-70,38,共4页Journal of Inspection and Quarantine

摘　　要：环介导等温扩增是一种新的核酸扩增技术,具有简单、快速、特异性强的特点。针对多种食源性致病菌和病毒的环介导等温扩增检测方法已建立了起来,现在主要是发展成为发展中国家一些高发传染病的快速诊断方法,今后环介导等温扩增和微流体等技术的联用有望推动基因床边诊断的实现。本文针对环介导等温扩增技术过去、现在的应用和未来的发展方向展开了论述。Loop-mediated isothermal amplification （LAMP） is an established nucleic acid amplification method offering a rapid, accurate, and cost-effective diagnosis of infectious diseases. This technology has been developed for a variety of pathogens, including bacteria and viruses. The current focus on LAMP methodology is to explore LAMP as a diagnostic system in resource-limited laboratories in developing countries, where many fatal tropical diseases are endemic. The combination of LAMP and novel microfluidic technologies may facilitate the realization of genetic point-of-care testing systems to be used in the near future. This review described the historical, current, and future developments of such technologies.

关 键 词：环介导等温扩增 病原菌 

分 类 号：R446.5[医药卫生—诊断学]